摘要
目的:近来研究显示,心力衰竭是由于神经内分泌细胞因子系统的激活及由此而介导的心室重塑的过程;其中血浆肿瘤坏死因子-α(TNF-α)是最重要的神经内分泌细胞因子之一。文中建立异丙基肾上腺素(ISO)诱导SD大鼠充血性心力衰竭模型,观察TNF-α变化并探讨其意义。方法:应用大剂量异丙基肾上腺素(170 mg/kg)对70只SD大鼠皮下注射2次,建立大鼠充血性心力衰竭模型,对照组10只大鼠皮下注射等渗盐水。18周后进行血流动力学检测2组大鼠心率、左心室收缩压、左心室舒张末期压、左心室内压最大上升和下降速率,取血利用放免法检测TNF-α,并取心肌观察病理形态学特征。结果:与对照组比较,模型组大鼠心率、左心室舒张末期压显著升高(P<0.01),左心室收缩压、左心室内压最大上升和下降速率显著下降(P<0.01),左心室质量指数明显增加(P<0.01),TNF-α显著升高(P<0.01)。光镜下显示模型组大鼠心肌细胞肥大,部分心肌细胞有嗜酸变性和(或)坏死,心肌间质结缔组织增生,心室重构明显。结论:大剂量异丙基肾上腺素诱导充血性心力衰竭的作用可能与TNF-α升高有关。
Objective： Recent studies have suggested that congestive heart failure（CHF） is related to neuroendocrine system and cytokines,and its activation contributes to myocardial remodeling and leads to the development and progression of CHF.Tumour necrosis factor-α（TNF-α） is one of the most important factors.This study intends to establish the animal model of isoproterenol（ISO）-induced CHF in rats and to study the changes of serum TNF-α.Methods：Male SD rats were randomly allocated to the ISO-induced CHF group（n=50） receiving two subcutaneous injections of 170mg ISO per kilogram of body and the healthy control group（n=10） receiving two subcutaneous injections of 0.25ml normal saline.At 18th week,the hemodynamic parameters including HR,LVSP,LVEDP and ±dp/dtmax were assessed.TNF-α in plasma were all detected.Results：Compared with the control group,the LVSP、±dp/dtmax significantly decreased and the HR,LVEDP enlarged notably in CHF group（P〈0.01）.Also the mass ratio of left ventricle to body and the concentration of TNF-α increased distinctly（P〈0.01）.Conclusion：Activation of plasma TNF-α observed in high-dose isoproterenol-induction rat model of CHF.
出处
《医学研究生学报》
CAS
2009年第12期1260-1263,I0010,共5页
Journal of Medical Postgraduates
基金
南京军区南京总医院科研基金资助项目(批准号:Q2008042)
