摘要
目的通过建立DSS诱导的恢复期UC小鼠模型,探讨青春双歧杆菌和嗜酸乳杆菌维持UC缓解的作用。方法 6～8周龄雌性BABL/c小鼠50只,体重(20.0±2.0)g,随机分为5组:正常对照组、嗜酸乳杆菌、青春双歧杆菌组、柳氮磺胺吡啶组和生理盐水对照组,每组10只。除正常对照组外,采用自由饮用2.5%DSS(相对分子质量3.6万～5万)7d建立小鼠UC模型,并从实验第1d开始,给予青春双歧杆菌、嗜酸乳杆菌(浓度均为2×108CFU/10g)和SASP(15mg/10g)灌肠治疗17d,观察小鼠一般情况、粪便隐血,计算疾病活动指数(DAI);实验结束时处死动物,测量全段结肠长度、行组织病理学检查并进行组织学评分。结果青春双歧杆菌、嗜酸乳酸杆均能维持实验性结肠炎的缓解,从第10d开始其DAI积分均明显小于生理盐水对照组(P<0.05);而且青春双歧杆菌有利于体重恢复,在第11d开始体重迅速增加;嗜酸乳酸杆菌在组织学改善方面效果更显著。结论青春双歧杆菌和嗜酸乳酸杆菌能有效地维持实验性结肠炎的缓解,而且两者的作用有所差别。
Objective To establish DSS-induced convalescent ulcerative colitis（UC） models and then investigate the effect of B.adolescentisand L.acidophilus on UC.Methods 50 specific pathogen free,（20±2）g,6-8 week-old BALB/c female mice were randomly allocated in 5 groups：normal control group（N）,L.acidophilus-treated group（L）,B.adolescentis-treated group（B）,SASP-treated group（S）,NS control group（NS）.With the exception of normal control mice,40 BABL/c mice were induced by 2.5% DSS water for 7 days free drinking and 10 days＇ distilled water.The mice were given 0.2ml B.adolescentis/L.acidophilus/SASP/NS via gavage once a day for 17 days,while normal controls let untreated.The animal were recorded daily both weight,fecal trait and bleeding（o-toluidine）,and then disease active index（DAI） was accounted.The animals were killed on 18th day morning.The whole colon（from the colo-cecal junction to the anus）was isolated for examination of length and pieces for hematoxylin and eosin stain,and histology scores were measured.Results B.adolescentis and L.acidophilus could relieve pathogenetic condition of DSS-induced mice.The DAI and histology scores of mice treated with probiotics were much lower than those did not from day 10（P〈0.05）.B.adolescentis was found more effective on protecting weight loss from 10th day,while L.acidophilus better on mucosa protection.Conclusion The B.adolescentis and L.acidophilus can maintain remission of UC,and the effect of the two stains are little different.
出处
《当代医学》
2010年第22期29-31,共3页
Contemporary Medicine
关键词
溃疡性结肠炎
恢复期
双歧杆菌
嗜酸乳酸杆
治疗
ulcerative colitis
convalescent
bifidobacterium
lactobacillus
treatment
