摘要
目的:建立以反相高效液相色谱法测定大鼠血浆及胃肠道内容物、黏膜中酮洛芬浓度的方法。方法:色谱柱为Hypersil C18,流动相为甲醇-50mmol·L-1磷酸二氢钾缓冲液(pH3.5,52∶48,V/V),检测波长为258nm,柱温为30℃,流速为1mL·min-1。结果:酮洛芬在各生物样本中检测浓度范围内线性关系良好(r为0.9926～0.9999);平均绝对回收率为83.76%～99.43%,平均方法回收率为86.41%～115.68%;日内RSD小于9.85%,日间RSD小于14.84%。结论:本方法准确、快速、灵敏,可用于生物样品中酮洛芬的浓度测定。
OBJECTIVE： To establish an RP-HPLC method for the determination of plasma, gastrointestinal content and mucosa concentration of ketoprofen in rats. METHODS： The separation was performed on Hypersil C18 chromatographic column. The mo bile phase consisted of methanol-50 mmol.L^-1 potassium dihydrogen phosphate buffer （pH 3.5,52：48, V/V） with a detection wavelength of 258 nm. The column temperature was 30℃ and the flow rate was 1 mL.min^-1. RESULTS： Good linear relationships were achieved when the detection concentrations of ketoprofen were within the range （r=0.992 6-0.999 9）. The average absolute recovery rate was 83.76%-99.43%, the average methodological recovery rate was 86.41%-115.68%, the intra-day RSD was less than 9.85% and the inter-day RSD was less than 14.84%. CONCLUSION： This method is accurate, rapid, sensitive and satisfying enough to be applied to measure the concentration of ketoprofen in biological samples.
出处
《中国药房》
CAS
CSCD
北大核心
2010年第1期35-37,共3页
China Pharmacy
