摘要
目的:研究牛蒡多糖的提取条件和对小鼠肠道菌群的调节作用。方法:采用单因素试验和正交试验优化牛蒡多糖提取工艺。15只小鼠随机分为空白对照组和牛蒡多糖低、高剂量组,每组5只,经口灌胃给药,灌胃容积为10ml/kg,1次/d,连续14d。给药结束后,分别采集小鼠粪便,检查肠道肠杆菌,乳杆菌和双歧杆菌。结果:牛蒡多糖优化提取条件为:温度80℃,固液比1:10(m/V),时间60min,提取两次。与空白对照组相比,牛蒡多糖组乳杆菌与双歧杆菌数量均显著增加(P<0.05),肠杆菌数量变化不明显(P>0.05)。结论:牛蒡多糖能有效调节小鼠肠道菌群,增殖乳杆菌和双歧杆菌数量。
Extraction conditions of burdock polysaccharide （BPS） were optimized by conducting single factor experiments and the following orthogonal test, and modulation effects of BPS on normal intestinal microflora of ICR mice were studied. The optimum conditions were as that burdock were extracted with hot water at 80℃ with the ratio of solid-liquid 1：10 for 60 min and extracted twice. The amounts of Bifidobacterium and Lactobacillus in mice of BPS treated groups significantly increased as compared with those in the blank group （P 0.05） , but the number of E.coli, Enterococcus was not significantly changed （P 0.05）.
出处
《食品科学》
CAS
CSCD
北大核心
2009年第23期428-431,共4页
Food Science
基金
北京市教委科技发展项目(KM200711417001)
关键词
牛蒡多糖
提取
小鼠
肠道菌群
burdock polysaccharide
extraction
mice
intestinal flora
