摘要
通过受精卵原核显微注射的途径，产生带有可诱导型丙肝病毒非结构蛋白３基因（ｎｏｎｓｔｒｕｃｔｕｒａｌ３ｇｅｎｅ）的重组载体的转基因小鼠。采用ＰＣＲ和基因组Ｓｏｕｔｈｅｒｎｂｌｏｔ杂交方法，在２１只新生鼠中筛选到３只建立者小鼠。其中２只与非转基因小鼠进行交配，分别得到８只和６只Ｆ１代小鼠。进而对其基因组ＤＮＡ进行检测，发现其中４只为转基因阳性个体。这些小鼠的获得，为ｎｓ３转基因品系的建立和ＮＳ３蛋白的生物学作用研究准备了条件。
To generate transgenic mice, the recombinant plasmids inserted the ns3 gene of hepatitis C virus were introduced into the male pronuclei of mouse fertilizer eggs by microinjection. By using polymerase chain reaction(PCR) and Southernblot hybridization method we obtained three mice(Founders) and four mice(F1) harbouring the ns3 gene among 21 offsprings born by the pseudopregnant mice and 14 individuals generated from the Founders mated with other nontransgenic mice, respectively. These transgenic mice would be helpful for the development of ns3 transgenic mice line and the study for the biological function of NS3 protein in vivo.
出处
《癌变．畸变．突变》
CAS
CSCD
1998年第4期193-198,共6页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金
关键词
丙肝病毒
转基因小鼠
NS3
Non-structural 3 gene
Hepatitis C Virus
Transgenic Mice
