摘要
目的对转基因注射过程中的基因装液方法进行改良。方法预先从显微注射针背部装入石蜡油至直管部,再使用可编程微量注射仪从注射针针尖倒吸入基因液。结果基因液顺利装入显微注射针,后续进行显微注射操作的压力状态稳定,避免溶液回吸。结论本法简便有效地将注射用基因液装入注射针,确保基因液注入原核,有利于提高转基因注射的成功率。
Objective To improve the method for loading DNA fragment solution into the microinjection pipette during transgenic microinjection. Method Sterile mineral oil was pre-loaded into the un- pulled section of the microinjection pipette from its back before DNA fragment solution was sucked into the pipette tip by programmable microinjector IM 300. Result DNA fragment solution was loaded into microinjection pipette successfully and easily. The balance pressure during microinjection process became steady and the withdrawal of DNA solution from the tip-opening was avoided. Conclusion DNA fragment solution can be loaded into the microinjection pipette conveniently and simply by our method where the programmable microinjector is used. And it also ensures that the DNA fragment solution can be injected into pronuclei with every movement of injecting operation, resulting a raise of success-rate in generating the transgenic animals.
出处
《福建医科大学学报》
2008年第5期419-421,共3页
Journal of Fujian Medical University
基金
福建省重点科技项目资助(2005Q008)
关键词
动物
遗体修饰
小鼠
转基因
显微注射
基因转移技术
animals, genetically modified
mice, transgenic
microinjections
gene transfer techniques
