摘要
目的探讨花椰菜芥子碱的抗辐射作用。方法通过HPLC法测定花椰菜芥子碱的含量,并以果蝇为材料,将纯化后的花椰菜芥子碱加入果蝇培养基中,观察芥子碱对紫外辐照后果蝇的繁殖率、性别比、突变率等影响,以考察花椰菜芥子碱活性提取物的辐射防护效果。结果测得花椰菜芥子碱硫氰酸盐的浓度为4.429μg/ml。紫外辐照后的果蝇在培养基中添加了浓度为0.29075mg/ml的芥子碱硫氰酸盐,果蝇繁殖率上升了27.083%,雌雄性别比下降了23.457%,突变率下降了97.202%,生长周期恢复正常。结论花椰菜芥子碱具有良好的辐射防护效果。
Objective To investigate the antiradiation effect of sinapine in Drosophita. Method Sinapine in cauliflower was quantified by HPLC, and added in Drosophila culture substrate. We observed the effect of sinapine from cauliflower on reproductive rate, sex ratio, mutation rate of Drosophila after UV radiation. Results Sinapine in cauliflower contained 4.429 μg/ml thiocyanate. After adding sinapine thiocyanate 0.29075 mg/ml to the media,the reproductive rate of UV irradiated Drosophila culture increased 27.083%, the sex ratio （female/male） and mutation rate declined 23.457% and 97.202% respectively, and the growth cycle became normal again. Conclusion Cauliflower’s sinapine thiocynate has quite favorable anti-radiation function.
出处
《营养学报》
CAS
CSCD
北大核心
2009年第6期588-590,共3页
Acta Nutrimenta Sinica
基金
江西省教育厅科技基金项目(NoGJJ08311)
江西省抚州市科技局基金项目,抚财教[2008]85号
关键词
花椰菜
芥子碱
果蝇
辐射保护
cauliflower sinapine drosophila radiation-defendable
