摘要
目的探讨创伤后应激障碍(PTSD)大鼠蓝斑(LC)神经元核受体-糖皮质激素受体(GR)表达的变化。方法成年健康雄性Wistar大鼠60只,使用无连续单一应激(SPS)方法建立PTSD大鼠模型,分别取SPS处理后24h、4d、7d、14d和28d大鼠蓝斑组织,同时取正常蓝斑组织(非SPS刺激)作为对照,应用尼氏染色观察蓝斑形态,采用免疫组织化学、免疫印迹和RT-PCR方法分别观察及检测各组蓝斑神经元GR表达的变化,并进行图像分析和统计学处理。结果GR分布在蓝斑神经元核上,GR的表达24h、4d、7d逐渐上调,14d、28d恢复性下调,但仍然高于对照组(P<0.05)。结论SPS处理后,蓝斑GR出现短暂上调,随后下调,提示PTSD大鼠蓝斑神经元核受体GR表达的变化可能直接参与PTSD持续精神症状的发生发展过程。
Objective To investigate expression changes of nuclear receptors-glucocorticoid receptors （GR） in the locus ceruleus neurons of posttraumatic stress disorder（PTSD） like rats. Methods Sixty adult healthy male Wistar rats were enrolled in the experiment. PTSD-like rat model was established by single prolonged stress （SPS）. There was no SPS stimulation in the control group and the rats of the other five groups were undisturbedlv raised for 24 hours, 4 days,7 days, 14 days and 28 days respectively after SPS stimulation. The expressions of glueocorticoid receptors in the locus ceruleus nucleus of all groups were detected with Nissl staining, immunohistochemistry, Western blotting and PCR, and image analysis and statistical analysis were performed. Results GR was distributed in the nucleus of coeruleus neurons, GR expression was showed after 24 hours, 4 days, 7 days treatment and gradually increased, restorative downregulation was seen after 14 days and 28 days, but still high（ P 〈 0.05）. Conclusion After SPS, the GR locus ceruleus temporarily increased and then lowered, suggesting that PTSD rat locus coeruleus neurons in nuclear receptor-GR expression changes may directly involve in the continuing spirit of PTSD symptoms occurred in the development process.
出处
《解剖学报》
CAS
CSCD
北大核心
2009年第6期873-876,共4页
Acta Anatomica Sinica
基金
国家自然科学基金资助项目(30600341)
关键词
创伤后应激障碍
蓝斑
核受体
糖皮质激素受体
免疫印迹法
大鼠
Posttraumatic stress disorders
Locus ceruleus
Nuclear receptors
Glucocorticoid receptors
Westeru blotting
Rat
