摘要
目的观察茵陈、白茅根、紫草等中药合剂茵白汤正丁醇提取液对α-萘异硫氰酸酯(ANIT)所致小鼠急性黄疸模型的退黄降酶作用。方法将小鼠随机分为正常组、模型组、阳性对照组(茵栀黄注射液)和茵白汤正丁醇提取液高、中、低剂量组。各治疗组小鼠连续灌胃给药7d后,ANIT诱发黄疸模型,48h后取血,分离血清,以血清肝功能指标以及肝脏组织病理学的改变为观察指标。结果与模型组比较,阳性对照组、茵白汤正丁醇提取液低剂量、中剂量组的丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)、总胆红素(T-BiL)、间接胆红素(I-BiL)和碱性磷酸酶(ALP)均显著下降(P<0.05);与阳性对照组比较,茵白汤正丁醇提取液低剂量、中剂量给药组ALT、AST、ALP、T-BiL、I-BiL均差异无显著性(P>0.05),肝脏病理组织学检查表明,茵白汤正丁醇提取液低剂量、中剂量给药组能明显减轻肝细胞变性、坏死和肝小胆管增生。结论茵白汤正丁醇低剂量、中剂量提取液具有降低实验性胆汁淤积小鼠血清胆红素、转氨酶和改善肝脏组织损伤的作用。
Objective To investigate the action of the n-butanol extract from Capillary Wormwood Herb, Rhizoma imperatae ,Radix Lithospermi Root of Sinkiang Amebia and so forth on reducing enzyme and receding jaundice in a jaundice model of mice induced by α-isothiocyanate （ANIT）. Methods Mice were randomly divided into six groups ： group A as normal control, group B as model control, group D, E, F treated with low, moderate and high dosage of ＂EXTRAC＂, respectively, and group E was given with＂ yinzhihuang injection＂ as positive control. Mice were p. o. treated for consecutively 7 d,except group A and B, then were given with ANIT to induce jaundice. After 48 h, the blood was withdrawn and serum was separated. The serum content of ALT,AST and ALP and pathological changes of liver tissue were observed. Results Compared with group B, ALT, AST, ALP, T-BiL, I-BiL in group C and D group obviously dropped （ P 〈 0.05 ） ; Compared with group C, those in group D group did not change significantly （ P 〉 0.05 ） ; It was found that ＂Extract＂ at a middle and high dose notably alleviated liver cell degeneration,necrosis and bile duct proliferation. Conclusion ＂ Extrac＂ has the action of reducing serum bilirubin and transaminase and improving liver tissue injury of experimental eholestasis in mice.
出处
《医药导报》
CAS
2010年第1期16-18,共3页
Herald of Medicine
基金
牡丹江医学院科研项目(基金编号:B200832)
