摘要
目的建立微生物法测定药品与食品中叶酸含量的方法,解决液相色谱不能测定食品化合物形式叶酸含量的不足,为孕妇叶酸日摄入量的统计提供理论依据。方法利用酪乳酸杆菌对叶酸生长的依赖性,测定其菌液光密度值,得到叶酸含量。结果检测3个种类的样品,得到了批内、批间、回收率的数据,并与液相色谱的检测结果做了比较。结论微生物法稳定可靠,比液相色谱法应用更广。
OBJECTIVE To explore a method to determine the folic acid content in medicament and foods by bioassayo to resolve the problem that folic acid content of foods can＇t be determined by HPLC, and to provide a way to calculate the ingestion of folic acid per day for pregnant woman. METHODS Utilizing the growth of Lactobacillus Casei （L.C） depending on folic acid to determine bacterial suspension＇s absorbency, the content of folic acid is obtained. RESULTS Through examining three kinds of samples, the coefficient variations of within-run, between-run and the rate of recovery have been got, and the result of the HPLC＇s was compared. CONCLUSION Bioassay is more stable and applicable than HPLC.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第12期1016-1019,共4页
Chinese Journal of Modern Applied Pharmacy
基金
浙江省医学科学院青年科研基金资助课题(A50708Q)
浙江省卫生高层次创新人才培养、浙江省151人才培养
