摘要
为研究8-烷基小檗碱同系物在体外对糖代谢的影响,采用与人肝细胞表型相似的HepG2细胞,检测24 h后培养液中葡萄糖消耗量,用MTT法监测细胞增殖的情况.结果表明:8-烷基小檗碱同系物在中糖(11.1mmol/L)状态下可使HepG2细胞的葡萄糖消耗量有不同程度的增加,其中以8-十六烷基小檗碱最为显著.MTT结果显示8-烷基小檗碱同系物对HepG2细胞的增殖有显著的抑制作用.8-烷基小檗碱同系物随着其烷基碳链的延长,其降糖效果越来越明显.
Aim： To investigate the glycometabolism of 8-Alkylberberine in vitro. Methods： HepG2 cells similar to human hepatic cells were used to test the glucose consumption（GC）, MTT assay was used to monitor the proliferation of the HepG2 cells. Results：In moderate high glucose concentration （11. 1 mmol/L）, 8-Alkylberberine could increase the glucose consumption of HepG2 cells, and 8-cetyl-berberine was the most evident. 8-Alkylberberine could also inhibit the proliferation of HepG2 cells. Conclusion： With the extension of carbon chain, the glucose-lowering effect of 8-Alkylberberine become increasingly evident.
出处
《西南师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第6期155-157,共3页
Journal of Southwest China Normal University(Natural Science Edition)
基金
重庆市科委自然科学基金资助项目(CSTC
2008BB5257)
重庆市科委"十一五"新药创制重大专项资助项目(CSTC
2008AA5021)
发光与实时分析重庆市重点实验室
开发基金资助项目(LRTA_2008CQ003)
