摘要
目的研究小檗碱能否通过刺激胰岛素分泌或直接作用于肝细胞而产生降糖作用。 方法检测HepG2细胞 2 4h培养液中葡萄糖的消耗量。另外检测 βTC3细胞胰岛素的释放量。 结果小檗碱 5～ 10 0 μmol/L可使HepG2细胞的葡萄糖消耗量增加 32 %～ 6 0 % (P <0 .0 0 1) ,与 1mmol/L二甲双胍相比无显著差异 ;小檗碱的降糖效能随着培养液中葡萄糖浓度的升高而降低 ;胰岛素对小檗碱的降糖作用没有明显的影响。小檗碱没有刺激βTC3细胞胰岛素分泌的作用。 结论小檗碱能通过肝细胞发挥非胰岛素依赖的降糖作用 。
Objective To study whether berberine (BER) is able to exert glucose-lowering effect on hepatocytes or stimulate the secretion of insulin. Methods For glucose consumption studies, the amounts of glucose disappeared from the culture medium of HepG2 cells within 24h were determined.βTC3 cells were also used to monitor insulin secretion. Results The glucose concentrations decreased significantly by BER 5 ～ 100μmol/L, while glucose consumption increased by 32% ～ 60%(P<0.001) with BER. The glucose-lowering effect of BER was similar to that of metformin. It declined as glucose concentrations increased independently on insulin. βTC3 cells were not stimulated by BER. Conclusion BER is able to exert glucose-lowering effect on hepatocytes which is insulin independent and similar to that of metformin, but has no effect on insulin secretion.
出处
《上海第二医科大学学报》
CSCD
2001年第5期425-427,共3页
Acta Universitatis Medicinalis Secondae Shanghai
基金
国家自然科学基金资助项目 (39870 31 1 )
