摘要
目的通过对比检测探讨不同血清胰岛素免疫检测系统存在的差异性及对临床判断胰岛功能的影响。方法选取51例患者，包括正常糖耐量13名，空腹血糖受损8名，糖耐量受损9名及2型糖尿病21名，采集血清样品152份。采用4种不同的检测方法平行测定血清胰岛素水平，其中放射免疫法1种、化学发光法2种和电化学发光法1种；另外再从21名2型糖尿病患者中选择14名患者行精氨酸刺激试验。结果4种方法检测血清胰岛素结果的相互间相关系数在0．81～0．99之间；检测数值之间的差异均显著（P〈0．01），但此差异对糖尿病临床诊断无显著影响。另外，在精氨酸刺激试验中，4种检测结果的趋向性表现一致，对临床判断胰岛功能无显著差异。结论不同的胰岛素检测方法既存在高度相关性也存在显著差异，4种不同的胰岛素检测方法对于临床判断患者胰岛β细胞分泌功能没有显著影响。
Objective To analyze the discrepancy among different serum insulin immunoassay systems and its clinical impact on the evaluation of islet β-cell secretary function. Methods Fifty-one patients were selected in the study, including 13 patients with normal glucose tolerance, 8 with impaired fasting glucose, 9 with impaired glucose tolerance and 21 with type 2 diabetes. Totally 152 serum samples were measured simultaneously for insulin levels by four different immunoassay systems, one radioimmunoassay, two chemiluminescence immunosaasys, and one immunoassay. 14 patients were chosen from 21 type 2 diabetes, and were performed with arginine stimulation test. Results Though the correlation coefficients between different assays ranges from 0.81 to 0.99, serum insulin levels measured by the four assay methods showed significant difference from each other （P〈0. 01 ）. However, those differences had no significant impact on the clinical management. Also in monitoring insulin in the arginine stimulation test, insulin levels of different serum samples resulting from four assays came up with almost same consistent tendency, which made no significant impact on the evaluation of β-cell function. Conclusion Serum insulin measured by four immunoassay methods present with significant difference though highly correlated with each other. However, those differences may have little impact on the clinical evaluation of patients with diabetes.
出处
《中华内分泌代谢杂志》
CAS
CSCD
北大核心
2009年第6期622-625,共4页
Chinese Journal of Endocrinology and Metabolism
关键词
胰岛素测定
放射免疫分析
化学发光法
电化学发光法
可比性
Insulin assay
Radioimmunoassay
Chemiluminescence immunosaasys
Electrochemilumines -cence immunoassays
Comparability
