摘要
以大豆子叶节为外植体,应用农杆菌介导法,将抗虫(cry 1Iem)基因转化大豆,筛选标记为bar基因。经Gl-ufasinate筛选,获得大量抗性植株。对转基因T0、T1、T2代植株进行PCR检测,初步证明cry1Iem基因已经整合到大豆基因组中。对T2代PCR阳性植株幼嫩豆荚,采用圆盘分隔法接入初孵幼虫,进行初步的抗虫性检测,得到1株具有明显抗虫效果和7株抗虫效果较好的转基因植株。
Contyledonary nodes were used as explants, and insect resistant gene cryllem was transformed into soybean via Agrobacterium-mediated method. Bar served as selection marker, and glufasinate was used in explants transformation to screen resistant shoots. Lots of resistant plants were obtained. With PCR detection of T0 , T1 , T2 generation, insect resistant gene cryllem was proved to be transformed into soybean preliminarily. Furthermore, immature pods of PCR positive plants in T2 generation and newly hatched larvaes were introduced, and initial insect resistant assay was made by disk method. Result suggested that one transgenic plant had an obvious resistant effect, and another seven plants proved to have some effect.
出处
《大豆科学》
CAS
CSCD
北大核心
2009年第6期959-963,共5页
Soybean Science
基金
国家高技术研究发展计划资助项目(2008AA10Z153)
转基因生物新品种培育重大专项资助项目(2008ZX08004-003)
东北农业大学创新团队资助项目(190214)
