摘要
目的:探讨不同标准对荧光原位杂交技术(fluorescence in situ hybridization,FISH)诊断膀胱癌的敏感度和特异性的影响。方法:选择20例健康人为正常组,计算FISH检查正常阈值;选择143例血尿患者为病例组.经FISH检查、尿脱落细胞学检查,比较Urovysion膀胱癌探针试剂盒标准和正常阈值标准诊断膀胱癌的敏感度和特异性。结果:采用Urovysion标准和正常阈值标准对膀胱癌的诊断敏感度分别为73.1%和100%,均较尿脱落细胞学检查明显增高,三者对膀胱癌诊断的特异性分别为90.0%、86%和100%。Urovysion标准与尿脱落细胞学联合检查时对膀胱癌诊断的敏感度明显升高,差异具有统计学意义(P<0.01)。结论:FISH检查较尿脱落细胞学检查诊断膀胱癌的敏感度显著提高,相比Urovysion标准,正常阈值更适合FISH诊断膀胱癌的标准。FISH与尿脱落细胞学联合检查能显著提高膀胱癌的诊断敏感度。
Objective：To find out if the sensitivity and specificity in the diagnosis of bladder cancer by fluorescenee in situ hybridization （FISH） is correlated with various standards. Methods： 20 patients without disease were tested with normal threshold for the FISH as normal group. All 143 patients with hemaluria were investigated by FISH and cytology respectively. We will apply standards adopted by Urovysion bladder cancer kit and the the nor maI threshold standards, and compare the sensitivity and specificity of the diagnosis of bladder cancer by various standards. Results：The sensitivity of diagnosing bladder cancer by Urovysion standard and the normal threshold standard is 73.1% and 100% respectively .and both are higher than that of cytology, the specificity of three diag nosis for bladder cancer is 90.0%, 86%, 100% respectively. The sensitivity of diagnosing bladder cancer would rise when both results were taken consideration meanwhile. The difference has statistically significant （ P 〈0 . 01）Conclusions： The sensitivity of diagnosing bladder cancer by FISH is higher tban by cytology. The normal threshold will be more suit for diagnosing bladder cancer by FISH than Urovysion standard, the sensitivity of diagnosis of bladder cancer will be elevated when both examines were taken.
出处
《临床泌尿外科杂志》
北大核心
2009年第12期889-891,894,共4页
Journal of Clinical Urology
基金
卫生部科研项目(WKJ2007-3-001)
关键词
膀胱癌
荧光原位杂交技术
诊断
bladder cancer
fluorescence in situ hybridization
diagnose
