摘要
目的:研究重庆土家族人群无关个体的15个STR基因座(CSF1PO、D13S317、D16S539、D18S51、D19S433、D21S11、D2S1338、D3S1358、D5S818、D7S820、D8S1179、FGA、TH01、TPOX、vWA)的遗传多态性。方法:应用AmpFlSTR Identifiler荧光标记复合扩增系统对115名重庆土家族无关个体血样DNA进行15个STR基因座的复合扩增,用ABI310遗传分析仪对扩增产物进行检测,用GeneScan、GenoTyper软件进行基因分型,统计计算15个STR基因座的群体遗传学参数。结果:15个STR基因座均符合Hardy-Weinberg平衡,杂合度在0.634 8～0.887 0之间,累积匹配概率和累积非父排除率分别为3.12×10-17和0.999 997 688 3。结论:15个STR基因座的累积匹配概率和累积非父排除率较高,适用于法医学亲子鉴定和个人识别。
Objective： To investigate the genetic polymorphism of 15 short tandem repeat （STR） loci （ CSF1PO, D 13S317, D 16S539, D 18S51, D 19S433, D21S 11, D2S 1338, D 3 S 1358, D5 S 818, D7S 820, D 8S 1179, FGA, THO 1, TPOX and vWA ） in Chongqing Tujia population. Methods： DNA samples from 115 Tujia unrelated individuals in Chongqing were screened with the use of AmpF1STR Identifiler PCR Amplification Kit and ABI310 Analyzer.The relative fragment＇s lengths of PCR products were calculated by gene scan analysis software and afterward genotyped by genotype software. Results： All the 15 loci met Hardy-Weinberg equilibrium.The heterozygosity （H） were ranged from 0.6348 to 0.8870. The combined probability of matching （Pm） and power of exclusion （PE） were determined to be 3.12 × 10-17 and 0.999 997 688 3. Conclusion： A higher measured value could be obtained for both the combined probability of matching and power of exclusion： The 15 STR loci can meet the needs of the paternity testing and individual identification in forensic medicine.
出处
《重庆医科大学学报》
CAS
CSCD
北大核心
2009年第11期1499-1502,共4页
Journal of Chongqing Medical University
