摘要
目的:建立细辛药材不同部位GC指纹图谱及α-蒎烯、1、8-桉叶素、甲基丁香酚3种成分同时测定的定量分析方法。方法:利用毛细管气相色谱法对采集的6批细辛药材地上部分、根及根茎进行指纹谱分析;采用外标一点法,同时测定了来源于我国8个省34批细辛商品药材中α-蒎烯、1、8-桉叶素、甲基丁香酚含量。结果:以39个共有峰为指标,建立了细辛不同部位气相色谱指纹谱。GC法测定α-蒎烯、1、8-桉叶素、甲基丁香酚含量,进样量在2.58~46.44ng、2.292~32.088ng、3.7768~151.872ng范围内呈良好的线性关系,回归系数分别为0.9990、0.9990、0.9997,平均回收率为95.35%(RSD=2.38%)、96.11%(RSD=3.08%)、94.89%(RSD=2.71%)。结论:GC法建立的细辛药材不同部位指纹图谱及含量测定方法,可为细辛药材不同来源与不同部位的质量评价提供科学依据。
Objective:To establish GC Fingerprint of Herba Asari in different parts and a quantitative analysis method for simultaneous determination of α - pinene, 1 8 - cineole and methyl eugenol of Herba Asari. Methods : Using capillary gas chromatography on the collected Herba Asari 6 batches in part on the ground, roots and rhizomes for fingerprint analysis ; and use of external standard method simultaneous determination of α - pinene, 1 8 - cineole and methyl eugenol of 34 batches Herba Asari from the eight provinces in China. Results: To establish GC Fingerprint of Herba Asari in different parts of 39 common peaks for indicators. Good linearlty of α -pinene, was showed within the range of 2.58 ~ 46.44ng( r =0. 9990), 1 8 -cineole within the range of 2. 292 -32. 088ng( r = 0.9990) and methyl eugenol within the range of 3. 7768 ~ 151. 872ng( r = 0.9997 ). The average recovery were 95.35% ( RSD = 2.38% ), 96. 11% ( RSD = 5.08% ), 94. 89% (. RSD = 2.71% ) respectively. Conclusion: The methods of GC Fingerprint and assay of Herba Asari in different parts can provide the scientific basis for quality assessment.
出处
《中华中医药学刊》
CAS
2009年第12期2612-2615,共4页
Chinese Archives of Traditional Chinese Medicine
基金
辽宁省教育厅资助项目(2009A486)