摘要
采用光镜和电镜技术，观察雏鸵鸟睾丸的显微和超微结构；采用免疫组织化学方法对NPY在睾丸内的表达进行研究，探明NPY在睾丸内的表达规律。光镜下可见，雏鸵鸟睾丸表面有一层由坚韧的结缔组织构成的白膜，它向睾丸实质内形成的结缔组织不发达，不形成睾丸纵隔和睾丸小隔；雏鸵鸟的曲精小管直径为85～90μm，生精上皮由支持细胞和1～2层生精细胞构成，此时精原细胞已开始分裂增殖，可见明显的分裂相，初级精母细胞数量少，体积大。电镜下可见，精原细胞胞质内有少量线粒体、游离核糖体和内质网；初级精母细胞富含线粒体、游离核糖体、内质网和溶酶体；支持细胞与精原细胞之间界限清晰，胞质内含较多游离核糖体，其他细胞器不发达；间质细胞的胞核形状不规则，内含较多脂滴。免疫组化显示，NPY在曲精小管周围、睾丸小血管周围、精母细胞和睾丸间质内均有表达，在睾丸小血管周围和精母细胞内表达量较高，而在曲精小管周围和睾丸间质内表达量较低。
This study aimed at observing the anatomy traits, microstructure and ultrastructure of the testes of the ostrich chicks and investigating the distribution of neuropeptide Y（NPY）immunoreactivity positive substance. Light and transmission electron microscopical methods were used to observe the morphological structure of ostrich testes. The location of NPY in the testes of the ostrich chicks was studied with immunohistochemical method, SABC. The results of the present study were as follows：there was albuginea composed of tenacious connective tissue on the sur face of the ostrich testes,but the connective tissue in the testes was not abundant, and there were not mediastinum testis and lobules of testis in ostrich testis. The diameter of the seminiferous tubule of ninety days ostrich was 85-90 μm. The seminiferous epithelium was composed of sustentacular cells and one or two layers spermatogenic cell. The spermatogenous cells began to cleavage and generation,and the phase of cleavage was saw obviously. The primary spermatocytes was bigger,but few and were smaller the nuclei. There were less mitochondria, free ribosome and endoplasmie reticulum in the intracytoplasm of the spermatogenous cells, and the nuclear pores were obvious. There were abundant cell organs in the intracytoplasm of the primary spermatocytes. The sustentacular cells contained more free ribosome,but other cell organs in them were not abundant. There was clear margin between the sustentacular cells and the spermatogenous cells. The shape of leydig cells was irregular, and the number of lipid droplet was major in the leydig cells. The results of immunohistochemistry test showed NPY immunoreactivity positive substance was distributed in the periphery of seminiferous tubule and small vessels of the testes, spermatocyte and interstitial tissue of testis.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第12期1599-1602,1606,共5页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30471249)
