摘要
目的探讨2型糖尿病、糖耐量减低者内脏和皮下脂肪组织的内脂素基因表达及与相关代谢指标的关系。方法研究对象均为汉族人群,对照组23例,无糖尿病家族史;糖耐量减低组(IGT组)18例;2型糖尿病组(T2DM组)22例。同时测量血压,计算体质量指数(BMI)及腰臀比(WHR),测定空腹血糖(FBG)、总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)、糖化血红蛋白(HbA1c)、空腹胰岛素(Fins)等指标,并计算稳态模型胰岛素抵抗指数(HOMA-IR)。半定量RT-PCR法检测脂肪组织内脂素基因的表达水平。结果T2DM组网膜脂肪组织的内脂素基因表达量高于IGT组和对照组(P均<0.01)。IGT组与对照组网膜脂肪组织的内脂素基因表达量差异无统计学意义(P>0.05)。对照组、IGT组及T2DM组皮下脂肪组织的内脂素基因表达量无统计学差异(P>0.05)。肥胖/超重组网膜脂肪组织的内脂素基因表达量高于体质量正常组(P<0.01)。各组网膜脂肪组织的内脂素基因表达量均高于对应的皮下脂肪组织的表达(P均<0.01)。相关分析中,网膜脂肪组织内脂素基因表达与WHR相关(P<0.05);与年龄、BMI、SBP、DBP、FINS、FBG、HbA1C、HOMA-IR均无相关性(P>0.05),而皮下脂肪组织内脂素基因表达则与TC相关(P<0.05)。结论内脂素在汉族人群T2DM、肥胖者中表达增加,且主要在内脏脂肪组织中表达,在维持葡萄糖稳态平衡中发挥作用。
Objective To investigate the difference of visfatin mRNA expression in abdominal subcutaneous and omental adipose tissues of type 2 diabetes and impaired glucose tolerance（the IGT group） and to explore the relationship between visfatin mRNA expression and indexes of correlation metabolism.Methods The subjects were Chinese Han population,including 23 controls with no family history of diabetes（the control group）,18 patients with impaired glucose tolerance（the IGT group） and 22 with type 2 diabetes（T2DM group）.The blood pressure,FBG,TC,TG,HDL-C,LDL-C,HbA1c,and Fins were determined,and（T2DM BMI,WHR,and HOMA-IR were calculated.Semi-quantitative RT-PCR was used to determine visfatin mRNA expression in adipose tissues.Results Visfatin mRNA expression of omental adipose tissues in the T2DM group was higher than that in the IGT and control groups（P〈0.01）,but was not significantly different between the IGI group and the control group（P〉0.05）.There was no statistical difference in visfatin mRNA expression of subcutaneous adipose tissues among the three groups（P〉0.05）.Visfatin expression of omental adipose tissues in the fat/overweight group was higher than that in the control group（P〈0.01）.Visfatin mRNA expression of omental adipose tissues was higher than that of subcutaneous adipose tissues（P〈0.01）.Correlated analysis showed that visfatin expression of omental a adipose tissues had a relation to WHR（P〈0.05）,but not to age,BMI,SBP,DBP,FINS,FBG,HbA1C,and HOMA-IR.While visfatin mRNA expression of subcutaneous adipose tissues was related to TC（P〈0.05）.Conclusion Visfatin expression increases in both T2DM patients and fat people,mainly in the omental adipose tissues,and plays effects in maintaining the balance of glucose homeostasis.
出处
《山东大学学报（医学版）》
CAS
北大核心
2009年第11期6-8,13,共4页
Journal of Shandong University:Health Sciences
