摘要
目的研究肿瘤抑制基因nm23在大肠癌发生发展中的作用,并探讨其与大肠癌转移的关系。方法组织芯片与免疫组化SP法检测87例大肠癌、26腺瘤、30例正常组织中nm23的表达。结果nm23在大肠癌、腺瘤、正常组织表达阳性率分别为38%、0%、3.3%,癌与腺瘤、癌与正常组织差异有显著性(P<0.01),在高、中、低分化腺癌阳性率分别为20%、38.3%、43.3%,高、低分化组差异有显著性(P<0.05),在淋巴结有无转移组阳性率分别为40%、37.2%,但差异无显著性(P>0.05)。结论nm23高表达与癌细胞的增生可能有关,与低侵袭转移表型没联系。
Objective To study the role of tumor suppressor gene nm23 in the development of colorectal carcinogenesis and its relationship with colorectal cancer metastasis. Methods Expression of nm23 in 87 cases of colorectal cancer, 26 cases of adenoma and 30 cases of normal tissue was detected by Tissue microarray and S - P immunohistochemical methods. Results the expression rate of nm23 in colorectal cancer, adenoma and normal tissues were 38%, 0% and 3.3% respectively. There were significant differences between cancer and adenoma, cancer and normal tissues （ P 〈 0.05 ）, no significant difference between adenoma and normal tissue （ P 〉 0.05 ） ; positive rates at high, medium and poorly differentiated adenocarcinoma were 20%, 38.3%, 43.3% respectively, between high and poorly differentiated group there was significant difference （ P 〈 0.05 ）, while no significant difference between medium and poorly differentiated group （ P 〉 0.05 ）. In the group with lymph node metastasis positive rates was 40%, in the group without lymph node metastasis 37.2%, between the two groups there was no significant difference （ P 〉 0. 05 ）. Conclusion nm23 overexpression may be related with proliferation of cancer cells,which was not related with the low phenotype of invasion and metastasis.
出处
《南华大学学报（医学版）》
2009年第6期670-672,共3页
Journal of Nanhua University(Medical Edition)
基金
省卫生厅资助课题
编号B2008-067
关键词
大肠癌
NM23
淋巴结转移
增生
免疫组织化学
colorectal cancer
nm23
lymphnode-melastasis
hyperplasia
immunohistochemistry
