摘要
目的探讨产超广谱β-内酰胺酶(ESBLs)和AmpC酶肺炎克雷伯菌(以下简称肺炎克雷伯菌双阳性菌株)对医院常用消毒剂的抵抗性,为控制其在医院内传播提供实验依据。方法用75%乙醇、2%戊二醛、500 mg/L有效氯的健之素、安尔碘4种消毒剂对肺炎克雷伯菌进行悬液定量杀菌试验和生物被膜片杀灭试验,分别计算杀灭率。结果在悬液定量杀菌试验中,作用1 min和3 min时,4种消毒剂对肺炎克雷伯菌双阳性菌株的杀灭率较肺炎克雷伯菌双阴性菌株分别低5.0%～10.0%、0.5%～3.5%,但在作用5 min和10 min时的杀灭率均≥99%。在生物被膜片杀菌效果与浮游菌的悬液定量杀菌效果对比中,肺炎克雷伯菌双阳性菌株在作用1 min时,4种消毒剂在前者中的杀菌率比后者低10.0%～15.0%;作用5 min时,低5.5%～19.0%;作用10min时,低0.1%～5.5%;作用30 min,杀菌率均≥99%。结论作用时间短时,产ESBLs和AmpC酶肺炎克雷伯菌对消毒剂的耐受性比ESBLs和AmpC酶阴性肺炎克雷伯菌明显较强;延长作用时间,常用消毒剂完全可以杀灭产ESBLs和AmpC酶肺炎克雷伯菌。
Objective To explore resistance of extended-spectrum β-lactamase （ESBLs）and AmpC producing Klebsiella pneumoniae （double positive K. pneumonia） to commonly used disinfectants, so as to provide reference for the control of bacterial transmission. Methods Four different disinfectants： 75% ethanol, 2% glutaraldehyde, 500 mg/L available chlorine solution and compound iodine solution were used to test their efficacy on K. pneumoniae with suspension quantitative germicidal test and biofilm germicidal test, the killing rates of each disinfectant was calculated. Results In suspension quantitative germicidal test, the killing rate of 4 kinds of disinfectants to the double positive strains was 5.0% - 10. 0%, 0. 5% - 3.5% lower than the double negative strains with a lmin and 3 min contact time respectively, the killing rates were all ≥99% with a 5min and 10 min contact time. The killing rate of 4 kinds of disinfectants to double positive strains in biofilm germicidal test was 10. 0% - 15. 0%, 5.5% - 19.0% and 0. 1 % - 5.5% lower than that in suspension quantitative germicidal test with a 1 min, 5min and 10 min contact time respectively,the killing rates were all ≥99% with a 30 min contact time. Conclusion When contact time is short, the resistance of ESBLs and AmpC-producing K. pneumoniae to disinfectants are stronger than non-ESBLs and non-AmpC-producing K. pneumoniae; when contact time is prolonged, commonly used disinfectants can kill ESBLs and AmpC-producing K. pneumoniae.
出处
《中国感染控制杂志》
CAS
2009年第6期397-399,402,共4页
Chinese Journal of Infection Control
