摘要
目的通过观察白屈菜红碱(chelerythrine,CHE)对原代培养的人颊黏膜成纤维细胞(human buccal mucosa fibro-blasts,HBMFs)生长的影响,筛选出CHE对HBMFs生长的抑制时间和浓度。方法以人体颊黏膜组织块进行HBMFs的原代培养并进行分离纯化及鉴定。以CHE作为干预条件,MTT法测定不同浓度CHE作用于离体HBMFs24、48、72h后的OD值。根据吸光度值的大小判断细胞的生长抑制情况。结果成功培养并分离纯化鉴定了HBMFs。CHE作用24h后,除392μg/mL组OD值较对照组明显降低外(P<0.05),其余组均未见明显抑制作用;作用48h后,392μg/mL组的OD值为0.095±0.005,196μg/mL组的OD值为0.102±0.006,较对照组明显降低(P<0.05);作用72h后,除49.0μg/mL组与24.5μg/mL组OD值与对照组相比差异无统计学意义外,其余各组差异具有统计学意义(P<0.05)。结论成功培养并分离纯化鉴定了HBMFs,筛选出了能够抑制HBMFs生长的不同时间和浓度。
Objective To observe the growth inhibiting of the primary culture human buccal mucosa fibroblasts （HBMFs） after chelerythrine（CHE） excitement,and to screen the different density and time of HBMFs＇ growth inhibiting by CHE excitement. Methods To perform primary culture and to separate,purify and idensity the HBMFs. CHE was used to excite HBMFs with different dentisy and time（24,48,72h）and determined the optical density by MTT reduction assay. To judge HBMFs＇ growth inhibiting by the optical density. Results HBMFs were successfully cultured,separated,purified and identified. HBMFs were excited by CHE in 24h,only the optical density of group 392μg/mL degraded obviously compared with control group（P〈0.05） ,other groups could not find the growth inhibiting;in 48h, the optical density of group 392μg/mL was 0. 095 ± 0. 005, the optical density of group 196μg/mL was 0. 102±0. 006,which both degraded obviously compared with control group（P〈0.05） ;in 72h,the optical density had no obviously difference of group 49.0μg/mL and group 24.5μg/mL compared with control group,and it showed statistical significance in the other groups（P〈0.05）. Conclusion Successfully culture,separate,purify and identify the HBMFs,and screen the different density and time of HBMFs＇ growth inhibiting by CHE excitement.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第23期2924-2925,I0003,共3页
Chongqing medicine
基金
重庆市卫生局科研基金资助项目(2008-2-62)
