摘要
目的以人舌癌细胞株Tca8113为研究对象,探讨ABCG2在Tca8113的表达情况及意义,探索在人舌癌细胞株Tca8113中是否存在具有干细胞特性的SP细胞,为今后分离舌癌的SP细胞提供一定的实验依据及理论数据。方法用细胞免疫荧光,激光共聚焦和流式细胞仪检测Tca8113中的ABCG2表达情况。结果用细胞免疫荧光,激光共聚焦检测出绿色的荧光为ABCG2蛋白,流式检测显示ABCG2的表达为5%左右。结论人舌癌细胞株Tca8113中存在少量具有干细胞特性的SP细胞。在后续的研究中,可考虑利用SP细胞的特异性表型标记"ABCG2",将SP细胞从肿瘤中分离出来,进一步探讨肿瘤干细胞的生物学特性。
Objective To investigate the expression and significance of ABCG2 in human tongue cancer cell line Tca8113. To investigate the existence of side population cells,with the potency of stem cells,in human tongue cancer cell line Tca8113 ,and to lay experimental basis for further isolating the SP cells in tongue cancer. Methods ABCG2 expression of Tca8113 was detected by immunofluorescence and FACS. Results ABCG2 protein was detected virent fluorescence by immunofluorescence measuring. The expression rate of ABCG2 in Tca8113 was about 5% by flow cytometry measuring. Conclusion SP cells,cancer-stem like cells,exist in human tongue cancer cell line Tca8113. The phenotype ＂ABCG2＂ which labeled SP cells will be considered to be a specific marker to isolate SP cells from the tumor cells. Through the research of SP cells, we will further explore the biological characteristics of tumor stem cells.
出处
《重庆医学》
CAS
CSCD
北大核心
2009年第23期2922-2923,I0003,共3页
Chongqing medicine
基金
重庆市卫生局科研基金资助项目(2008-2-232)
重庆市卫生局科研基金资助项目(052153)
重庆市科委自然科学基金资助项目(CSTC.2005DB5241)
关键词
人舌癌
SP细胞
ABCG2
流式细胞术
human tongue cancer
side population cells
ABCG2
flow cytometry
