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腺病毒介导的白细胞介素10基因转染对呼吸机相关性肺损伤大鼠全身炎性反应的影响

Effect of adenoviral-mediated interleukin-10 gene transfection on systemic inflammatory responses in a rat model of ventilator-induced lung injury
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摘要 目的 探讨腺病毒介导的白细胞介素10(Ad-IL-10)基因转染对呼吸机相关性肺损伤大鼠全身炎性反应的影响。方法健康成年雄性SD大鼠30只,体重200~220g,随机分为5组(n=6):空白对照组(C组)、通气2h空白腺病毒组(B1组)、通气4h空白腺病毒组(B,组)、通气2hAd—IL-10基因转染组(A1组)和通气4hAd—IL-10基因转染组(A2组)。C组不做任何处理,B.组和B1组分别于麻醉后右心室腔内注射空白腺病毒0.1ml,A,组和A2组分别于麻醉后右心室腔内注射含IL-10基因的腺病毒0.1ml,滴度1×10^9pfu/ml,48h后行机械通气,维持气道压力25cmH2O各组于通气2h或4h时放血处死大鼠,采集腹主动脉血样5ml,测定血清IL-8和IL10浓度,观察肺组织病理学结果及超微结构。结果与C组比较,其余各组血清IL-8和IL-10浓度升高(P〈0.01);与B1组比较,B2组血清IL-8和IL-10浓度升高,A1组血清IL-8浓度降低,IL-10浓度升高(P〈0.05或0.01);与A2组比较,A1组血清IL-8和IL10浓度降低,B2组血清IL-8浓度升高,IL10浓度降低(P〈0.05或0.01)。B1组和B2组病理损伤程度严重,A1组和A2组病理损伤程度减轻,A2组损伤减轻程度更明显。结论Ad-IL-10基因转染可减轻呼吸机相关性肺损伤大鼠全身炎性反应,从而减轻肺损伤。 Objective To investigate the effect of adenoviral-mediated interleukin-10 (IL-10) gene transfection on systemic inflanmatory responses in a rat model of ventilator-induced lung injury. Methods Thirty adult male SD rats weighing 200-220 g were randomly divided into 5 groups ( n = 6 each) : group Ⅰ control (group C) ; group Ⅱ adenovirus without IL-10 gene + 2 h mechanical ventilation (MV) (group B1 ) ; group Ⅲ adenovirus without IL-10 gene + 4 h MV (group B2 ) ; group IV adenovirus with IL-10 gene + 2 h MV (group A1 ) and group Ⅴadenovirus with IL-10 gene + 4 h MV (group A2 ). In group Ⅱ-V the animals were mechanically ventilated (airway pressure = 25 cm H2O) and adenovirus was injected into right ventricle at 48 h before MV. Blood samples were taken from abdominal aorta at the end of 2 h or 4 h MV for determination of serum IL-8 and IL-10 concentrations. The animals were then killed. The puhnonary specimens were obtained for examination of uhrastructure with light and electron microscope. Results Mechanical ventilation significantly increased serum IL-8 and IL-10 concentrations in a duration-dependent manner in group Ⅱ- V as compared with group C. Transpnhnonary administration of adenoviral-mediated interleukin-10 gene significantly decreased sermn IL-8 concentration and increased serum IL-10 concentration and reduced ventilator-induced lung injury after MV in group A1 and A2 as compared with group B1 and B2 .Conclusion Adenoviral-madiated IL-10 gene transfection can attenuate the systemic inflammatory response and reduce ventilator-induced lung injury.
作者 马武华 陈鸶
出处 《中华麻醉学杂志》 CAS CSCD 北大核心 2009年第10期932-934,共3页 Chinese Journal of Anesthesiology
基金 广东省自然科学基金项目(06300032)
关键词 腺病毒科 白细胞介素10 转染 呼吸 人工 呼吸窘迫综合征 成人 全身 炎症反应综合征 Adenoviridae lnterleu kin-10 Transfection Respiration, artificial Respiratory distress syndrome, adult Systemic inflammatory, response syndrome
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参考文献10

  • 1Metnitz PG, Barrens C, Fischer M, et al. Antioxidant status in patients with acute respiratory distress syndrome. Intensive Care Med, 1999,25: 180-185. 被引量:1
  • 2Ranieri VM, Suter PM, Tortorella C, et al. Effect of mechanical ventilation on inflammatory mediators in patients with acute respiratory distress syndrome : a randomized controlled trial. JAMA, 1999,282 : 54-61. 被引量:1
  • 3Lista G, Colnaghi M, Castohli F, et al. Lung injury and ventilatory strategies. Pediatr Med Chir, 2003,25 : 35-41. 被引量:1
  • 4Takata M, Abe J, Tanaka H, et al. Intraalveolar expression of tumor necrosis factor-alpha gene during conventional and high-frequency ventilation. Am J Respir Crit Care Med, 1997,156:272-279. 被引量:1
  • 5王颖,岳子勇,李文志,杨万超,张莉莉.异丙酚减轻大鼠机械通气所致肺损伤[J].国际麻醉学与复苏杂志,2007,28(3):200-204. 被引量:5
  • 6Walder B, Fontao E, Totsch M, et al. Time and tidal volume-dependent ventilator-induced lung injury in heahhy rate. Eur J Anaesthesiol, 2005, 22 : 785-794. 被引量:1
  • 7杨昭云,徐军美,姜金玉,郭亮,廖用琼,钱其军.人白细胞介素-10和Bcl-2基因重组腺病毒经冠状动脉转染大鼠心肌的可行性[J].中华麻醉学杂志,2006,26(11):1001-1004. 被引量:6
  • 8hnanaka H, Shimaoka M, Matsuura N, et al. Ventilator-induced lung injury is associated with neutrophil infiltration, macrophage activation, and TGF- beta 1 mRNA upregulation in rat lungs. Anesth Analg, 2001,92 : 428- 436. 被引量:1
  • 9Hou J, Baichwal V, Cao Z. Regulatory elements and iranscfiption factors controlling basal and cytokine-induced expression of the gene encoding intercellular adhesion molecule 1. Proc Natl Acad Sci USA, 1994, 91 : 11641-11645. 被引量:1
  • 10Smith WB, Gamble JR, Clark-Lewis I, et al. Chemotactic desensitization of neutrophils demonstrales inlerleukin-8 ( 11,-8)-dependent and 11,-8- independent mechanisms of transmigration through cytokine-activated endothelium, Immunology, 1993, 78:491-497. 被引量:1

二级参考文献10

  • 1姚媛媛,王焱林,王成夭.异丙酚对内毒素诱导的急性肺损伤的保护作用[J].实用医学进修杂志,2004,32(3):147-151. 被引量:2
  • 2Barr E, Carroll J, Kalynych AM, et al. Efficient catheter-mediated gene transfer into the heart using replication-defective adenovirus. Gene Ther,1994,1:51-58. 被引量:1
  • 3Miao W, Luo Z, Kisis RN, et al. Intracoronary adenovirus-mediated Akt gene transfer in heart limits infart size following ischemia-reperfusion injury in vivo. J Mol Cell Cardiol, 2000,32:2397-2402. 被引量:1
  • 4Lee LY, Patel SR, Hackett NR, et al. Focal angingen therapy using intramyocardial delivery of an adenovirus vector coding for vascular endothelial growth factor 121. Ann Thorac Surg, 2000,69:14-23. 被引量:1
  • 5Lin H, Parmacek MS, Morle G, et al. Expression of recombinant genes in myocardium in vivo after direct injection of DNA. Circulation, 1990,82: 2217-2221. 被引量:1
  • 6Russell WC. Update on adenovirus and its vectors. J Gen Virol, 2000,81 : 2573-2604. 被引量:1
  • 7Wang S, Banm BJ, Yamano S, et al. Adenoviral-mediatod gene transfer to mouse salivary glands. J Dent Res. 2000,79:701-708. 被引量:1
  • 8Suzuki M, Suzuki S, Yamamoto N, et al. Immune responses against replication-deficient adenovirus inhibit ovalbumin-specific allergic reactions in mice. Hum Gene Ther, 2000, 11:827-838. 被引量:1
  • 9Peters AH, Drumm J, Ferrell C, et al. Absence of germline infection in male mice following intraventricular injection of adenovirus. Mol Ther,2001,4:603-613. 被引量:1
  • 10赵晶,罗爱伦,Joern Heine,Siegfred Piepenbrock.不同细胞分离制备方法下异丙酚对中性粒细胞呼吸爆发作用的影响[J].中华麻醉学杂志,2002,22(8):471-473. 被引量:11

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