摘要
目的:建立HPLC柱后衍生化法测定前列腺素E1(PGE1)脂肪乳中PGE1的含量。方法:采用固相萃取技术对样品进行预处理,使用Kromasil C18色谱柱(300 mm×4.6 mm,10μm),流动相为0.01 mol.L-1磷酸盐缓冲液(pH6.3)-乙腈(75∶25),流速1.0 mL.min-1,柱后反应液为1 mol.L-1氢氧化钾溶液,流速0.50 mL.min-1,反应温度为60℃,前列腺素B1(PGB1)为内标,检测波长为280 nm。结果:PGE1浓度在2.4～9μg.mL-1范围内线性关系良好,平均回收率(n=9)分别为97.2%(RSD=1.4%),98.4%(RSD=0.6%),97.8%(RSD=1.8%)。结论:此方法准确灵敏,可用于PGE1脂肪乳的质量控制。
Objective：To develop a post - column derivatization chromatographic method for the determination of prostaglandin E1 （PGEI）in lipid emulsion. Methods：Utilizing solid -phase extraction ,the analytical column was a Kromasil Cls（300 mm ×4. 6mm,10μm）. The mobile phase was a mixture of 0. 01 mol · L^-1 phosphate buffer acetonitrile（75： 25）at a flow rate of 1.0 mL · min ^-1. The post- column reaction mixture consisted of 1 mol· L^-1 potassium hydroxide flowing at 0. 5 mL· min^-1 , and the temperature was 60 ℃. The internal standards was prostaglandin B1 （PGB1 ）. The detection wavelength was at 280 nm. Results： The calibration curves of prostaglandin E1 was in good linearity over the range of 2. 4 - 9 μg · mL^-1, the average recoveries （ n = 9 ） were 97.2% （ RSD = 1.4% ） ,98.4% （ RSD = 0. 6% ）, 97.8% （ RSD = 1.8% ） respectively. Conclusion： The method is simple, rapid, reliable and can be used for the drug control.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2009年第11期1801-1803,共3页
Chinese Journal of Pharmaceutical Analysis
