摘要
对长白山红蚂蚁(Forimica aquilonia)的抗肿瘤进行活性研究。MTT比色法测定长白山红蚂蚁提取物对大鼠肝癌细胞dRLh84增殖抑制作用,荧光显微镜观察提取物作用于dRLh84细胞后的肿瘤细胞的细胞核变化。结果表明:长白山红蚂蚁乙酸乙酯提取物具有抑制dRLh84细胞增殖作用,且呈剂量-效应关系。乙酸乙酯提取物以乙酸乙酯和水进行液液萃取后,活性成分移动至乙酸乙酯相,证明活性物质为极性较小的物质。荧光显微镜观察结果表明,长白山红蚂蚁乙酸乙酯提取物能引起dRLh84细胞凋亡。
In order to evaluate the antitumor activity of red wood ants （Formica aquilonia） from Changbai mountain, ethyl acetate extract from this species of red wood ants and its ethyl acetate-soluble and water-soluble fractions were investigated for effects on proliferation and apoptosis of dRLh-84 cells using MTT assay and fluoroscopic observation, respectively. The ethyl acetate extract was able to inhibit the proliferation of dRLh-84 cells in a dose-dependent manner. Active components in the extract were transferred into ethyl acetate phase in ethyl acetate-water extraction due to low polarity so that the ethyl acetate phase also exhibited a significant inhibitory effect on the proliferation of dRLh-84 cells, which depended on dose with an IC50 of 60μg/ml. Fluoroscopic observation indicated that the extract had an obvious apoptosis-promoting effect on dRLh-84 cells.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第21期335-337,共3页
Food Science
基金
吉林农业大学与岩手大学共同合作研究项目
