摘要
目的：进一步研究茶多酚（ＴＰ）在体外细胞毒作用、体内抗肿瘤的作用。方法：噻唑兰（ＭＴＴ）体外试验法和灌胃给药体内抗瘤试验。结果：茶多酚对体外培养的人鼻咽癌细胞ＣＮＥ２、人肺腺癌Ａ５４９细胞和ＧＬＣ８２细胞及乳腺癌细胞ＭＣＦ７的半数抑制浓度（ＩＣ５０）依次为１０２７２μｇ／ｍｌ、３５７６μｇ／ｍｌ、７００μｇ／ｍｌ和６３１０μｇ／ｍｌ。体内灌胃给予ＴＰ１２５ｍｇ／ｋｇ、５０ｍｇ／ｋｇ和２０ｍｇ／ｋｇ·ｄ×１０天剂量下对小鼠肿瘤Ｌ２的抑瘤率依次为３１０６～３０９９％、４２０５～４９７５％和４５１２～４６７９％，Ｐ＜００５～００１。ＴＰ在１２５ｍｇ／ｋｇ、５０ｍｇ／ｋｇ和２０ｍｇ／ｋｇ·ｄ×１８天剂量下，对人肺癌裸鼠移植瘤的抑瘤率依次为２９７７％、３７４１％和４８３４％，后二者Ｐ＜００５～００１，有显著性意义。结论：茶多酚在体外显示细胞毒作用。
Purpose: To study cytotoxic effect and antitumor effect of tea polyphenoles (TP). Methods: MTT assay method and in vivo antitumor test were used. Results: IC50 of GTP on human NPC cell CNE2, human lumg adenocarcinoma cell A549 and GLC 82 as well as breast adenocarcinoma cell, MCF 7 were 102 72,35 76,70 0 and 63 10 μg/ml, respectivly. Under the dose of 1 25, 5 0, 20 0 mg/kg·d×10d, the inhibitory rates of TP on L2 in mice were 31 06～33 99%, 42 05～49 75% and 45 12～46 79% respectively. At the above dose the inhibtiory rates of TP against Xenograft of human lung adenocarcinoma in nude mice were 29 77%,37 41% and 48 34% separately. Conclusion: TP possessed in vitro and in vivo antitumor activities.
出处
《癌症》
SCIE
CAS
CSCD
北大核心
1998年第6期418-420,427,共4页
Chinese Journal of Cancer
基金
国家"九五"科技攻关研究项目
关键词
茶多酚
肺肿瘤
裸鼠
抗肿瘤作用
Tea polyphenols/pharmacology
Human lung cancer
Nude mice
