摘要
将采自辽宁兴城地区在生长期间具典型卷叶病症状的金星无核（Venus Seedless）葡萄品种休眠枝条,用RT-PCR检测4种葡萄卷叶伴随病毒（Grapevine leafroll-associated viruses,GLRaVs）,扩增得到了葡萄卷叶伴随病毒2号（GLRaV-2）和葡萄卷叶伴随病毒3号（GLRaV-3）两种病毒的主要外壳蛋白（major coat protein,CP）基因的完整序列（GenBank登录号分别为FJ786017和FJ786016）。这表明该葡萄植株受到了GLRaV-2和GLRaV-3辽宁分离物（GLRaV-2-LN和GLRaV-3-LN）的复合侵染。根据检测结果,克隆了GLRaV-2-LN基因组3′端CPm（minor capsid protein）、p19（19-kDa protein）和p24（24-kDa protein）基因（GenBank登录号分别为FJ786018、FJ786019和FJ786018）。序列分析表明,GLRaV-3-LN的CP基因全长942 nt,与已报道的国内外其它分离物CP基因全序列相比,核苷酸序列同源性为89.8%~91.8%,由此推导的氨基酸序列同源性为94.9%~97.4%。GLRaV-2-LN的CP、CPm、p19和p24基因全长分别为597 nt、672 nt、486 nt和618 nt。与国外报道的几个分离物的相应蛋白基因全序列相比,核苷酸序列同源性分别为88.3%~100.0%、78.7%~99.9%、75.1%~99.4%和87.5%~99.5%;由此推导的氨基酸序列同源性分别为92.9%~100.0%、89.2%~100.0%、73.9%~99.4%和89.3%~99.0%。
The dormant cutting of grapevine （Vitis vinifera cv. Venus Seedless） with typical leafroll symptom originated from Xingcheng region in Liaoning Province was detected for Grapevine leafroll-associated vi- ruses （GLRaVs） by RT-PCR. The results showed that two complete sequences of major coat protein （CP） gene of GLRaV-2 （GenBank accession number FJ786017） and GLRaV-3 （FJ786016） were obtained, which indicated that the grapevine plant detected was co-infected with GLRaV-2 and GLRaV-3. The genes encoding CPm, pl9 and p24 located in 3＇genomic RNA of GLRaV-2 Liaoning isolate （LN） were then cloned and se- quenced. The nucleotide sequence of GLRaV-3 CP was 942 nt, and sequence analysis showed that the identities of nucleotide sequence and deduced amino acid sequence among GLRaV-3-LN and other previously reported isolates including ten overseas and two domestic isolates were ranged from 89.8% to 91.8% and 94.9% to 97.4%, respectively. The nucleotide sequences of CP, CPm （ FJ786018 ）, p19 （ FJ786019 ） and p24 （FJ786018） of GLRaV-2-LN were 597 nt, 672 nt, 486 nt and 618 nt in length, respectively. The relative sequence analysis showed that the nucleotide identities of CP, CPm, p19 and p24 among GLRaV-2-LN and other isolates reported were ranged from 88.3% to 100.0%, 78.9% to 100.0%, 75.1% to 99.4% and 87.5% to 99.5%, and the identities of deduced amino acids were 92.9 to 100.0%, 89.2% to 100.0%, 73.9% to 100.0% and 89.3% to 99.0%, respectively.
出处
《植物病理学报》
CAS
CSCD
北大核心
2009年第5期458-465,共8页
Acta Phytopathologica Sinica
