摘要
采用ELISA方法、分光光度计法和组织学方法,系统研究了不同年龄小体鲟(Acipenser ruthenus)血清卵黄蛋白原(Vitellogenin,Vg)、血清钙离子(Calcium,Ca2+)含量与卵巢发育变化的关系。结果表明:血清Vg浓度、Ca2+浓度与卵巢的发育时期相关;在卵黄沉积期内,血清中Vg浓度与Ca2+浓度呈线性正相关(R2=0.9264);卵黄生成前期和沉积初期,血清中Vg浓度和Ca2+含量较低;卵黄沉积期内,卵母细胞由沉积初期的0.56mm增大到卵黄沉积末期的2.01mm,血清Vg质量浓度由110.65μg/mL上升到242.22μg/mL,血清Ca2+浓度由2.34mol/L增大到2.72mol/L。对同年龄的雌、雄小体鲟血清Vg浓度和Ca2+浓度测定表明,根据血清中Vg和Ca2+含量差别,可以区分出沉积期小体鲟的性别。
Sturgeon is an ancient family（Acipenseridae）of fishe close to the divergence fish that eventually evolved into terrestrial animals and those which evolved into modern teleost species.An enzyme immunoassay and flame photometry were used to measured the level of serum vitellogenin（Vg）and calcium of sterlet sturgeon（Acipenser ruthenus）,cultured in Fishery Academy of China Sturgeon Propagation of Technology and Engineering Center,Fangshan,Beijing,respectively.The assay,based on polyclonal rabbit and mouse antibody to purified Lv,was tested for specificity and applied on sturgeon broodstocks（age 1-7 years）.Serum Vg concentrations were correlated with total serum calcium and stage of ovarian maturity.Females in the previtellogenic ovarian stage and at the onset of yolk deposition had low Vg concentrations,but serum Vg and calcium increased after onset of yolk deposition in the oocytes.During vitellogenesis the oocytes diameter increased from 0.56 mm to 2.01 mm,and the serum Vg levels changed from110.65μg/mL to 242.22μg/mL and the serum Ca 2＋ levels increased from 2.34 mol/L to 2.72 mol/L.Total serum calcium exhibited a linear relationship with serum Vg concentration.Concentrations of both metabolites in serum discriminate previtellogenic and vitellogenic females,but fail to discriminate specific stage of ovarian vitellogenesis.The immunoassay for serum Vg or flame photometry for total plasma calcium can be utilized to segregate vitellogenic females in cultured sterlet sturgeon.
出处
《中国水产科学》
CAS
CSCD
北大核心
2009年第6期967-974,共8页
Journal of Fishery Sciences of China
基金
"十一五"国家支撑计划资助项目(2006BAD03B08)
黑龙江水产研究所基本业务费(2008HSYZX-SJ-04)
