摘要
对人工养殖施氏鲟卵巢发育的不同时期(1～8龄)进行了组织学观察,结果表明:1龄施氏鲟处于性分化早期,卵巢中卵原细胞形成增殖团;2～3龄鱼卵巢为I期,第1时相的初级卵母细胞直径10～60μm,核大,位于细胞中央,染色质均匀分布;4～5龄卵巢为Ⅱ期,第2时相小生长期的初级卵母细胞直径60～200μm,核内含有7～26个核仁;6～7龄卵巢Ⅲ期,第3时相大生长期的初级卵母细胞直径200～1600μm,卵母细胞内出现脂肪滴、卵黄颗粒和色素颗粒。细胞膜有完整的3层结构。8龄卵巢Ⅳ期,第4时相晚期初级卵母细胞直径1600～3300μm,细胞核膜消失,核仁溶解,核由中心向动物极受精孔移动;Ⅴ期卵巢的第5时相生殖细胞为成熟的卵细胞,直径3400～3750μm,从滤泡膜中释放到体腔内;Ⅵ期为产卵后的卵巢,以第2时相的卵母细胞为主。根据研究结果推测,施氏鲟卵巢再次成熟的周期为3～4年。
The process of ovarian development in artificially-bred Amur sturgeon(1-8 years old) was investigated histologically. It shows that in fish one year old, ovarian was made up of a lot of oogonium clusters and two or three years old, germ cells of phase I ovarium was primary gonocyte stage(10-60μm) or chromatin stage and had a large nucleus occupying the greater part of oocyte. The germ cells of phaseⅡ ovarium in four or five years were early growth period oocyteⅠ(60-200μm)or perinucleolus stage and had 7-26 nucleolus in them and phase Ⅲ ovarium in six or seven years were middle growth period oocyte I(200-1600μm). In this stage, oil droplets, yolk and pigment granules appeared at the periphery of the nucleus in a cytoplasm. The cell membrane had complete three layers. The germ cells of phase Ⅳ ovarium in eight years were late period oocytes I(1600-3300μm). The nuclear membrane disappeared and the nucleus dissolved. Oocytes at the most advanced stage had the nucleus moving to the micropylar region of the animal pole. The germ cells of phase Ⅴovarium were ripe oocytes(3400-3750μm) and were released from follicle to body cavity. After spawning, the phase Ⅵ ovarium had a few phase 2 ovum, so results show that the cycle of ripe ovarium again is about three or four years.
出处
《水产学报》
CAS
CSCD
北大核心
2004年第5期487-492,共6页
Journal of Fisheries of China
基金
科技部"十五"科技攻关计划(2001BA5050506)
关键词
施氏鲟
卵巢发育
组织学
Acipenser schrenckii
ovarian development
histology
