Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice
Construction and Application of Efficient Ac-Ds Transposon Tagging Vectors in Rice
摘要
Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches： （i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,
Transposons are effective mutagens alternative to T-DNA for the generation of insertional mutants in many plant species including those whose transformation is inefficient. The current strategies of transposon tagging are usually slow and labor-intensive and yield low frequency of tagged lines. We have constructed a series of transposon tagging vectors based on three approaches： （i） AcTPase controlled by glucocorticoid binding domain/VP16 acidic activation domain/Gal4 DNA-binding domain （GVG） chemical-inducible expression system; （ii） deletion of AcTPase via Cre-lox site-specific recombination that was initially triggered by Ds excision; and （iii） suppression of early transposition events in transformed rice callus through a dual-functional hygromycin resistance gene in a novel Ds element （HPT-Ds）, We tested these vectors in transgenic rice and characterized the transposition events. Our results showed that these vectors are useful resources for functional genomics of rice and other crop plants. The vectors are freely available for the community,
参考文献43
-
1Alonso JM, Stepanova AN, Leisse T J, Kim C J, Chen H, Shinn P et al. (2003), Genome-wide insertional mutagenesis of Arabidopsis thafiana. Science 301,653-657. 被引量：1
-
2Aoyama T, Chua NH (1997). A glucocorticoid-mediated transcriptional induction system in transgenic plants. Plant J. 11,605-612. 被引量：1
-
3Becker D, Lutticke R, Li M, Starlinger P (1992). Control of excision frequency of maize transposable element Ds in Petunia protoplasts. Proc. Natl. Acad. Sci. USA 89, 5552-5556. 被引量：1
-
4Christensen AH, Quail PH (1996). Ubiquitin promoter-based vectors for high-level expression of selectable and/or screenable marker genes in monocotyledonous plants. Transgenic Res. 5, 213-218. 被引量：1
-
5De Block M, Botterman J, Vanderwiele M, Dockx J, Thoen C, Gossele Vet al. (1987). Engineering herbicide resistance in plants by expression of a detoxifying enzyme. EMBO J. 6, 2513-2518. 被引量：1
-
6Enoki H, Izawa T, Kawahara M, Komatsu M, Koh S, Kyozuka Jet al. (1999). Ac as a tool for the functional genornics of rice. Plant J. 19, 605-613. 被引量：1
-
7Greco R, Ouwerkerk PBF, Taal A J, Favalli C, Beguiristain T, Puigdomenech Pet al. (2001a). Early and multiple Ac transpositions in rice suitable for efficient insertional mutagenesis. Plant Mol. Biol. 46, 215-227. Erratum in Plant Mol. Biol. 46, 763-764. 被引量：1
-
8Greco R, Ouwerkerk PBF, Sallaud C, Kohli A, Colombo L, Puigdomenech P et al. (2001b). Transposon insertional mutagenesis in rice. Plant Physiol. 125, 1175-1177. 被引量：1
-
9Greco R, Ouwerkerk PBF, de Kam RJ, Sallaud C, Favalli C, Colombo L et al. (2003). Transpositional behaviour of an Ac/Ds system for reverse genetics in rice, Theor. Appl. Genet, 108, 10-24. 被引量：1
-
10Ito T, Motohashi R, Kuromori T, Mizukado S, Sakurai T, Kanahara H et al. (2002). A new resource of locally transposed Dissociation elements for screening gene-knockout lines in silico on the Arabidopsis genome. Plant Physiol. 129, 1695-1699. 被引量：1
-
1李成涛,薛景珍,何朝族.转座子标签法在构建G^-细菌突变体库中的应用[J].微生物学杂志,2002,22(4):17-19. 被引量：3
-
2宁顺斌,王玲,宋运淳.一组基于RT-PCR的未知产物基因克隆技术及其在植物方面的应用[J].武汉植物学研究,1999,17(A09):31-38. 被引量：3
-
3胡英考.转座子标签法克隆分离植物基因的研究进展[J].生物技术通报,2003,19(2):18-21. 被引量：9
-
4陈双臣,张孝峰,刘星,曹福中,云兴福,邹志荣.植物转座子及其在番茄功能基因组的应用[J].生物技术,2005,15(6):83-85.
-
5高新起.转座子标签法克隆基因的改进——sAc/Ds双系统法[J].生物学通报,2000,35(9):31-32. 被引量：1
-
6曹月青,何正波,王中康,殷幼平,彭国雄,夏玉先.丝状真菌基因功能研究的方法[J].应用与环境生物学报,2007,13(2):284-288. 被引量：9
-
7徐剑宏,洪青,汪婷,张小华,李顺鹏.转座子标签法突变呋喃丹降解菌CFDS-1[J].微生物学通报,2005,32(2):34-38. 被引量：3
-
8江树业,陈启锋,方宣钧,李维明,吴为人.植物基因分离方法及其评述[J].福建农业大学学报,2000,29(3):261-268. 被引量：4
-
9陈扬勋,张治国,路铁刚.无筛选标记转基因作物的研究进展[J].生物技术通报,2012,28(12):1-7. 被引量：5
-
10吴迪,刘斌,侯文胜,朱延明,韩天富.转座子标签法及其在大豆功能基因组研究中的应用前景[J].大豆科学,2007,26(2):254-258. 被引量：1
