摘要
目的构建mic2/CD99基因表达载体。方法通过PCR及双酶切方法,从Jurkat细胞基因组RNA中获得mic2/CD99全长基因编码序列,克隆到pcDNA3.1(+)质粒载体上,构建包含mic2/CD99全长基因载体,并从分子水平检测及验证。结果经PCR方法扩增出大小为585bp片段,序列测定其编码序列正确,酶切鉴定亚克隆序列正确。结论成功构建了mic2/CD99全长基因载体,为后续研究打下基础。
[ Objective ] To construct the CD99 gene eukaryotic expressing vector. [Methods] Full length cDNA of CD99 was acquired from jurkat cell line by RT-PCR . Design PCR primer with enzyme cleavage locus, get CD99 gene expression frame by PCR, then recombine to the vector pcDNA3.1 （＋）. The sequence of the PCR product was confirmed by enzyme cleaving and DNA sequencing analysis. [Result] The fragment of 585 bp was amplified and the sequence was correct by DNA sequencing analysis. [Conclusion] Eukaryotic expression vector pcDNA3.1（＋）-CD99 was successfully constructed.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第19期2927-2930,共4页
China Journal of Modern Medicine
基金
国家自然科学基金(No:30670812)
