摘要
目的了解临床尿液标本分离的60株大肠埃希菌消毒剂、灭菌剂耐药基因的存在情况。方法收集2006年1月-2008年10月从住院患者尿液中分离出大肠埃希菌60株,应用K-B纸片扩散法检测细菌对15种抗菌药物的敏感性;应用PCR及序列分析3种消毒剂、灭菌剂耐药基因(qacE△1t、ehA、merA)。结果60株大肠埃希菌除对亚胺培南、哌拉西林/他唑巴坦、头孢西丁、阿米卡星、庆大霉素有较高的敏感率(>70.0%)外,对其他10种抗菌药物的敏感率均较低(<50.0%);60株菌中42株检出qacE△1基因(70.0%),10株检出merA基因(16.7%),全部检出tehA基因,1号菌tehA基因测得序列与美国核酸库(GenBank)已登录的tehA基因序列不同,为新亚型。结论临床尿液标分离的大肠埃希菌70.0%携带qacE△1基因,未来细菌消毒剂的耐药可能是医院感染的主要因素之一。
OBJECTIVE To investigate the disinfectant and antibiotic resistance genotypes in 60 strains of Escherichia coli isolated from urine. METHODS Sixty strains of E. coli isolated from inpatients＇ urine were collected from Jan 2006 to Oct 2008. Antibiotic susceptibility tests for fifteen antibiotics were performed by Kirby-Bauer method. And three kinds of disinfectant and antibiotic resistance genes （qacE△1, tehA, merA）were analyzed by polymerase chain reaction （PCR） and DNA sequencing. RESULTS More than 70.0% of the sixty strains of E. coli were susceptible to imipenem, piperacillin/tasobactam, cefoxitin, amikacin and gentamicin, and less than 50.0% were susceptible to the other ten antibiotics. There were 42 strains with qacE△1 gene （70. 0%）, 10 strains with merA gene （16. 7%） and all strains with tehA gene. The sequence of the first strain was different from those reported in GenBank, so it was a new subtype. CONCLUSIONS There are 70% of E. coli strains isolated from urine samples with qacE△1 gen. And disinfectant resistance may be one of the main factors for hospital infection in the future.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第22期3007-3010,共4页
Chinese Journal of Nosocomiology
基金
江苏省实验诊断学重点实验室重大项目(XK200731)
关键词
大肠埃希菌
消毒剂
灭菌剂
基因
Escherichia coli
Disinfectant
Antibiotic
Gene
