摘要
目的了解耐甲氧西林金黄色葡萄球菌(MRSA)qacA/qacB(qacA/B)消毒剂耐药基因存在状况,并讨论其临床意义。方法收集221株临床分离的MRSA进行qacA/B基因检测。结果221株MRSA中qacA/B检出101株(45.7%),其中71株并作qacA基因特异性聚合酶链反应(PCR)检测,检出qacA基因20株(28.2%),而该71株qacA/B基因检出27株(38.0%),提示有7株为qacB基因。结论MRSA已出现高频度的qacA/B消毒剂耐药基因,用氯己定等消毒剂预防术后医院感染必须重新评估;现行的消毒剂或消毒方法的有效性,应引起我国医院消毒界的广泛重视;消毒剂耐药基因检测技术为细菌耐消毒剂的临床应用研究和分子流行病学研究等提供了具有实际应用价值的技术手段。
OBJECTIVE To investigate the qacA/B genes in meticillin-resistant Staphylococcus aureus （MRSA） and their significance. METHODS Totally 221 MRSA strains were clinically isolated. The genes of qacA/B were analyzed using polymerase chain reaction （PCR）. RESULTS From them 101 strains were found the qacA/B genes, the positive rate of qacA/B genes was 45.7%. And 71 strains were selected for qacA PCR detection, 20 （28.2%） were with qacA gene and 27 （38.0%） were with qaeA/B gene, suggesting that the seven strains be with qacB gene. CONCLUSIONS MRSA strains have＇ emerged the high-frequency qacA/B disinfectant resistance gene. Application Of chlorhexidine and other disinfectants to prevent postoperative nosocomial infections must be reassessed. The efficacy of existing disinfectants or disinfection methods should arouse our wider attention. Disinfectant resistance gene detection technology provides a practical means for the research of the disinfectantresistant bacteria and the clinical application of the molecular epidemiology research.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2008年第11期1522-1524,共3页
Chinese Journal of Nosocomiology
基金
杭州市余杭区科技局科研基金资助(2005-12)
