摘要
目的探讨应用脂蛋白脂酶基因（LPL）缺陷的杂合子小鼠作为制备高脂血症相关性急性胰腺炎的动物模型的可行性。方法野生型及LPL杂合子小鼠均分为实验组和对照组，每组又分为12、24h2个时点。实验组腹腔注射雨蛙肽（50ug/kg体重）7次，每次间隔1h。对照组同法注射等量生理盐水。检测各组小鼠血浆三酰甘油（TG）、淀粉酶水平，观察胰腺形态学改变并评分。结果 LPL杂合子小鼠对照组血TG为（3．55±0．27）mmol／L，显著高于野生型小鼠对照组的（0．94±0．18）mmol／L（P〈0．05）。杂合子小鼠实验组12h的血TG、淀粉酶水平分别为（3．55±0．27）mmol／L和（3685±484）U／L，均显著高于野生型小鼠实验组12h的（0．92±0．11）mmol／L和（2501±410）U／L（P〈0．05）。杂合子小鼠实验组12h的胰腺水肿、坏死、出血和炎细胞浸润的分值分别为3．94±0．21、3．94±0．21、1．84±0．25和1．84±0．25，均显著高于野生型小鼠实验组12h的3．06±0．01、2．52±0．51、0．46±0．22和0．58±0．38（P〈0．05）。结论LPL杂合子小鼠血TG中度升高且稳定，在雨蛙肽诱导下产生严重的急性胰腺炎，是研究高脂血症相关性急性胰腺炎发病机制的一种理想的动物模型。
Objective To establish an experimental animal model of hypertriglyceridemic （HTG） pancreatitis by using special lipoprotein lipase （LPL） deficient HTG heterozygous mice. Methods LPL deficient HTG heterozygous mice and wild type mice were divided into experiment group and control group, then each group was subdivided into 12 h,24 h subgroup. Acute pancreatitis （AP） was induced by caerulein intra-abdominal injection for 7 times （50 p.g/kg） at the interval of 1 h; the mice in the control group received same amount of normal saline. The serum level of TG, amylase was determined, and morphologic changes were scored. Results The serum level of TG in LPL deficient HTG heterozygous mice was （ 3.55± 0.27 ） mmol/L, which was significantly higher than that of wild type mice [ （ 0.94 ± 0. 18 ） mmol/L, P 〈 0.05 ]. The serum level of TG and amylase in heterozygous mice at 12 h was （3.55 ± 0.27）mmol/L and （3685 ± 484）U/L, which was significantly higher than those in wild type mice [ （ 0.92± 0.11 ） mmol/L and （ 2501 ± 410 ） U/L, P 〈 0.05 ]. The pancreatic tissue edema, necrosis, bleeding and inflammatory cell infiltration score was 3.94 ± 0.21,3.94± 0.21, 1.84 ± 0.25 and 1.84 ± 0.25 in heterozygous mice, which was significantly higher than that of wild type mice （3.06 ± 0.01, 2.52 ± 0.51,0.46 ± 0.22 and 0.58± 0. 38, P 〈 0.05 ）. Conclusions The serum level of TG was moderately and stably elevated in heterozygous mice. The mice developed more severe pancreatitis after caerulein induction, which was an ideal experimental model for study of mechanism of HTG pancreatitis.
出处
《中华胰腺病杂志》
CAS
2009年第5期340-342,共3页
Chinese Journal of Pancreatology
关键词
胰腺炎
高酯血症
脂蛋白酯酶
杂合子
Pancreatitis
Hypertriglyceridemia
Lipeprotein lipase
Heterozygote
