摘要
拟南芥遮光培养2.5d时,rfc3-1突变体黄化幼苗的下胚轴平均长度约比野生型植株黄化幼苗的下胚轴长27.5%。观察表明,相对于野生型复制因子C亚基3(replication factor C3,AtRFC3)基因突变体的下胚轴表皮细胞,特别是上部靠近子叶部分的表皮细胞,单细胞长度变长。将野生型RFC3基因转染到rfc3-1后,突变体恢复野生型表型,进一步说明RFC3在黄化苗的下胚轴伸长生长中有作用。
The average length of etiolated hypocotyl of rfc3-1 mutant was about 27.5% longer than that of the wild type plants under dark culture conditions for 2.5 days. The main reason is that mutation in RFC3 （replication factor C3） causes the length of single epidermal cell, especially the epidermal cell close to cotyledon becoming longer than that of the wild type. Transformation of wild type AtRFC3 gene into rfc3 mutant rescued the mutant phenotypes, suggesting that RFC3 plays a role in hypocotyl elongation of etiolated seedlings in Arabidopsis thaliana.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第10期991-994,共4页
Plant Physiology Communications
基金
国家自然基金项目(30970247)
湖南省高等学校科学研究重点项目(09A037)
作物种质创新与资源利用国家重点实验室培育基地开放重点项目
湖南农业大学稳定人才基金(07WD14)
关键词
拟南芥
RFC3
黄化苗
下胚轴
伸长生长
Arabidopsis thaliana
RFC3
etiolated seedling
hypocotyl
elongation
