摘要
以我国常见的刀额新对虾为研究对象,旨在获得其主要过敏原的氨基酸序列等基础数据,增加对虾类过敏原的认识和理解。分离和纯化了分子量为36 kD的虾主要过敏原蛋白,利用激光辅助解析/飞行时间质谱对其进行肽质量指纹图谱鉴定,并对鉴定的结果采用Mascot搜索引擎在NCBInr数据库上进行搜索。结果表明刀额新对虾主要过敏原蛋白与斑节对虾原肌球蛋白匹配分值最高为268,吻合肽段27条,序列覆盖率为65%;与其它无脊椎动物如腐食酪螨、衣鱼等的原肌球蛋白的序列覆盖率也很高,分别达到了51%和53%。这一结果不仅表明了刀额新对虾与其它甲壳类海产品过敏原存在着高度同源性,而且为其与甲壳类及其它无脊椎动物主要过敏原之间存在严重交叉反应的现象提供了理论依据。
Sand shrimps （Metapenaeus ensis ） were investigated in order to obtain the basic amino acid sequence data of its major allergen and to enhance the awareness of crustacean allergen. The major allergen protein of sand shrimps with the molecular weight：36 kD was extracted and separated. Then the molecular identification of the 36 kD protein by MALDI-TOF/MS was carried out and its peptide mass fingerprint was obtained. Finally, the figures obtained were identified by searching in the NCBInr database with the Mascot search engine. The results showed that the top one protein with the highest mowse value： 268 was the tropomyosin of giant tiger prawn（Penaeus monodon ）. There were 27 queries matched and the fraction of amino acid sequence coverages was 65 %. The matching scores and the sequence coverages were also high with other invertebrates including copramite （ Tyrophagus putrescentiae） and silverfish （Lepisma saccharina ）. The resuits described above not only elucidated that the allergen of Metapenaeus ensis had high homology with other crustacean allergens but also provided evidence to explain why the crustacean major allergens showed high crossreactivity with those of various invertebrates.
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第5期919-924,共6页
Periodical of Ocean University of China
基金
国家高技术研究发展计划项目(2006AA09Z427)
国家“十一五”科技支撑计划(2006BAD02A27)资助
