摘要
分离纯化金鲳鱼小清蛋白(parvalbumin,PV),并对其进行结构与性质分析。经Tris-HCl粗提、热处理、联合2次柱层析可从金鲳鱼肌肉中得到较高纯度的目的蛋白,经SDS-PAGE分析,其分子质量约10 kDa。蛋白免疫印迹显示,10 kDa处有阳性反应,进一步质谱鉴定确定其为金鲳鱼PV蛋白。PV蛋白结构分析表明,其为典型的α-螺旋结构,在248 nm处有紫外特征吸收峰,在350 nm处有内源荧光最大发射峰。PV蛋白性质分析表明,金鲳鱼PV蛋白热稳定较好,耐弱酸性、耐碱性。消化稳定性试验表明,胃蛋白酶可分解金鲳鱼PV蛋白,而金鲳鱼PV蛋白对胰蛋白酶具有抗性。
This study aimed to isolate and purify parvalbumin(PV)from Trachinotus ovatus.The structure and property of PV were also analyzed.The protein was obtained from the Trachinotus ovatus by Tris-HCl extraction,heat treatment,and twice of column chromatography.The SDS-PAGE result showed the molecular weight of the protein was about 10 kDa.The result of the Western blot showed that positive reaction at 10 kDa,which was further identified as PV by mass spectrometry.The structure analysis of PV showed that it contained typicalα-helix.The UV absorption peak at 248 nm was observed and the peak of intrinsic fluorescence was found at 350 nm.The characterization analysis of PV showed that it had thermal stability and was resistant to weak acids and alkalis.The digestion test showed that pepsin could decompose PV,but it was resistant under trypsin.
作者
邱惠
王新
段伟文
魏帅
孙钦秀
夏秋瑜
王泽富
韩宗元
刘书成
QIU Hui;WANG Xin;DUAN Weiwen;WEI Shuai;SUN Qinxiu;XIA Qiuyu;WANG Zefu;HAN Zongyuan;LIU Shucheng(College of Food Science and Technology,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Aquatic Products Processing and Safety,Guangdong Province Engineering Laboratory for Marine Biological Products,Guangdong Provincial Engineering Technology Research Center of seafood,Key Laboratory of Advanced Processing of Aquatic Product of Guangdong Higher Education Institution,Zhanjiang 524088,China;Southern Marine Science and Engineering Guangdong Laboratory,Zhanjiang 524025,China;Dalian Polytechnic University,Collaborative Innovation Center of Seafood Deep Processing,Dalian 116034,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2023年第12期215-220,共6页
Food and Fermentation Industries
基金
南方海洋科学与工程广东省实验室(湛江)(ZJW-2019-06)
广东普通高等学校海洋食品绿色加工技术研究团队(2019KCXTD011)
广东海洋大学博士科研启动项目(R20048)。
关键词
金鲳鱼
过敏源
小清蛋白
结构
稳定性
Trachinotus ovatus
allergen
parvalbumin
structure
stability
