摘要
目的建立浓缩六味地黄丸中马钱苷、芍药苷的含量同时测定方法。方法采用高效液相色谱法,色谱柱为Hypersil C18(200 mm×4.6 mm,5μm),Nova-PakC18 Guard-PakTM保护柱;流动相:0.05%磷酸水溶液-乙腈进行梯度洗脱;柱温:30℃;流速:1 mL/min。结果马钱苷在0.11～1.10μg范围内,进样量与峰面积线性关系良好(r=0.999 5);样品的平均回收率为99.2%,RSD为1.3%(n=5)。芍药苷在0.15～1.50μg范围内,进样量与峰面积线性关系良好(r=0.999 2);样品的平均回收率为100.4%,RSD为1.9%(n=5)。结论此方法简便、快速,可作为浓缩六味地黄丸中马钱苷、芍药苷的含量同时测定方法。
Objective To establish a determination method of loganin and paeoniflorin for Liuwei Dihuang pill. Method There were the stationary phase with Hypersil C18 （200 mm×4.6 mm 5 μm） Nova-PakC18 Guard-PakTM, 0.05% phosphoric acid solution（A）-acetonirtile（B）, The photodiode array detector at 233nm, column temperature 30℃ and the flow rate 1 mL/min in HPLC. Result For loganin, the calibration curve 0.999 5）, the average recovery of the method was linear over the range of 0.11-1.10 μg （r= was 100.1% , RSD =3.0% （n =5）. For paeoniflorin, the calibration curve was linear over the range of 0.15-1.50 μg （r=0.999 2）, the average recovery of the method was 100.7%,RSD=2.1%（n=5）. Conclusion The method is reliable and accurate, which can be used to determine the contents of loganin and paeoniflorin in Enriched Liuwei Dihuang pills.
出处
《湖南中医药大学学报》
CAS
2009年第5期35-37,41,共4页
Journal of Hunan University of Chinese Medicine
基金
湖南省教育厅课题(06C610)
湖南省中医药管理局课题(6201)
