摘要
目的:建立调经助孕颗粒中马钱苷的含量测定方法。方法:采用高效液相色谱法,色谱柱为SinoChromODS.BP(250mm×4.6mm,5μm),流动相为乙腈-0.1%磷酸水溶液(14:86),检测波长为240nm,柱温30℃。结果:马钱苷进样量在50.40~1008.00ng范围内与峰面积有良好的线性关系(r=0.9999),平均加样回收率为99.33%,RSD为1.41%(n=6)。结论:该方法快速、准确,操作简单,专属性强,可作为调经助孕颗粒中马钱苷的含量测定方法。
Objective: To establish a determination method for loganin in Tiaojing Zhuyun granules by HPLC. Methods: HPLC was used with a SinoChrom ODS-BP (250 mm×4.6mm,5μm) as the analytical column. The mobile phase was composed of acetonitrile-0.1% phosphoric acid solution ( 14: 86). The detection wavelength was set at 240 nm and the column temperature was 30℃. Resuits : The calibration curve of loganin was in a good linearity over the range of 50.40-1 008.00 ng( r = 0.999 9 ). The average recovery was 99.33 % (RSD = 1.41%, n = 6). Conclusion: The method is simple, feasible and reproducible, and can be used in the quality control of Tiaojing Zhuyun granules.
出处
《中国药师》
CAS
2014年第2期324-326,共3页
China Pharmacist