摘要
建立一台基于同步扫描相机的双光子激发荧光寿命显微测量系统,同步扫描相机的重复工作频率为76MHz,利用钛宝石飞秒激光器作为光源,通过可调延时器和标准具对扫描相机的时间分辨率、扫描速度以及非线性等进行标定.该系统的时间分辨率为9ps,非线性小于4%,量程为2.8ns.测量了荧光染料Rose Bengal(RsB)的荧光衰减曲线,通过最小二乘法对荧光的衰减曲线进行拟合,得到RsB的荧光寿命为763ps,与标准荧光染料对比一致.
A two-photon excitation fluorescence lifetime spectrometer based on a synchroscan streak camera was presented. The spectrometer was calibrated using a Titanium： Sapphire femtosecond laser in conjunction with a custom-made synchronized streak circuit, tunable delay generator and a set of etalons. Experimental results show that the temporal resolution of this spectrometer is 9 ps, nonlinearity is less than 4%, and the sweep range is 2. 8 ns. Fluorescence decay profile of fluorescent dye Rose Bengal （RsB） is recorded with the spectrometer. Nonlinear least square fitting of the decay profile gives that the lifetime of RsB is 763 ps, consistent with standard fluorescence dyes.
出处
《深圳大学学报（理工版）》
EI
CAS
北大核心
2009年第4期331-336,共6页
Journal of Shenzhen University(Science and Engineering)
基金
国家自然科学基金资助项目(60627003)
广东省科技计划资助项目(2008a060205003)
关键词
荧光显微技术
荧光寿命
双光子激发
同步扫描相机
扫描速度
时间分辨率
fluorescence microscopy
fluorescence lifetime
two-photon excitation
synchroscan streak camera
sweep speed
temporal resolution
