摘要
报道了用自由基引发剂(AAPH)引发氧化,反相高效液相色谱法分离卵磷脂(Phos-phatidylcholine,PC)及其氢过氧化物(PC-OOH),获得纯品PC-OOH,以及50℃自动氧化后,用硅胶柱层析分离亚油酸甲酯(MethylLinoleate,ML)及其氢过氧化物(ML-OOH),而获得标准ML-OOH的方法。
In this paper, the preparation methods of Phosphatidylcholine hydroperoxide (PC OOH) and Methyl linoleate hydroperoxide (ML OOH) are reported. We use water soluble free radical initiator (AAPH) to oxidate Phosphatidylcholine (PC) for a period of time, and PC OOH was isolated from the mixture by reverse phase HPLC. The Methyl Linoleate (ML) was placed in dark at 50℃ for several days, and ML OOH was acquired by silica column chromatographic isolation.
关键词
卵磷脂
氢过氧化物
亚油酸甲酯
氢过氧化物
制备
phosphatidyl choline hydroperoxide(PC OOH)
methyl linoleate hydroperoxide(ML OOH)
Preparation