摘要
本研究通过提取土壤总DNA,利用特异引物进行PCR扩增和变性梯度凝胶电泳(DGGE),研究了不同品种水稻对稻田土壤氨氧化细菌和氨氧化古菌群落结构组成的影响。结果显示:稻田土壤具有丰富的氨氧化细菌和氨氧化古菌,且氨氧化古菌种类更多;不同品种水稻氨氧化细菌群落结构组成差异较大,其中以"天A/Km71"、"闽优1027"和"Km62/1027"3个品种相似性较高,且明显不同于其余3个品种;而氨氧化古菌群落结构组成在不同土层间表现出较大差异,其中以"天A/Km71"和"Km62/1027"的表土与根际土氨氧化古菌群落组成差异最大。研究表明不同水稻品种及土壤层次对氨氧化微生物群落结构组成具有一定影响,证明氨氧化微生物尤其是氨氧化古菌在稻田土壤生态系统中占有重要地位。
Community structures of ammonia-oxidizing bacteria and ammonia-oxidizing archaea in paddy soils of different rice cultivars were investigated by PCR denaturing gradient gel electrophoresis for soil DNA production. The results show that ammonia-oxidizing bacteria and ammonia-oxidizing archaea in paddy soils are rich, with more sorts in ammonia-oxidizing archaea than ammonia-oxidizing bacteria. The effect of rice cultivars on ammonia-oxidizing bacteria is strong. The community structures of ammonia-oxidizing bacteria are similar among the ＂TianA/Km71＂, ＂Minyou1027＂ and ＂Km62/1027＂ rice cultivars, but different among the other three rice cultivars. Soil site effect on ammonia-oxidizing archaea is very obvious, with the highest differences in ammonia-oxidizing archaea community structures between bulk and rhizosphere soils of ＂TianA/Km71＂ and ＂Km62/1027＂ rice cultivars. The results demonstrate that different rice cultivars and soil site influence the community structures of ammonia-oxidizing microbial and ammomia-oxidizer, which, especially ammonia-oxidizing archaea, are important for the paddy soil ecosystems.
出处
《中国生态农业学报》
CAS
CSCD
北大核心
2009年第6期1211-1215,共5页
Chinese Journal of Eco-Agriculture
基金
国家自然科学基金项目(40801097)
福建省青年人才创新项目(2007F3018)
福建省科技重点项目(2008N0110)资助
关键词
水稻
氨氧化细菌
氨氧化古菌
根际土
群落结构
PCR-DGGE
Rice
Ammonia-oxidizing bacteria
Ammonia-oxidizing archaea
Rhizosphere soil
Community structure
PCR-DGGE
