摘要
从16个多年种植Bt棉花的田块抽取土壤样本。采用PCR和ELISA方法测定了土壤试样中Bt基因序列片段和杀虫蛋白的残留和累积效应。结果表明,以土壤总DNA为模板不能扩增出棉花内标准基因和cry1A基因预期大小片段。连续种植Bt棉花可以在土壤中低剂量残留Bt杀虫蛋白,但不会导致Bt毒蛋白在土壤中累积。
Soil samples were collected from within sixteen fields where insect-resistant transgenic cotton encoding the crylA gene had been grown and subsequently incorporated into soil by post-harvest tillage for multiple years. The crylA gene sequence fragments and Bt insecticidal protein in these samples （collected after the last season tillage） were explored using PCR amplification and enzyme-linked immunosorbent assay （ELISA） method, respectively. Soil total DNA could be gotten in high quality, but we could not get any PCR amplification result of cotton internal standard gene or crylA gene. The results from ELISA indicated that repeated agricultural using of transgenic Bt cotton expressing CrylA insecticidal protein could persist extremely low levels of Bt protein in soil. However, the persistence of Bt protein in soil did not result in accumulation by post-harvest tillage in multiple seasons.
出处
《棉花学报》
CSCD
北大核心
2008年第1期79-80,F0003,共3页
Cotton Science
基金
国家重点基础研究发展规划项目(001CB109004)
关键词
BT棉花
土壤
BT杀虫蛋白
残留
transgenic Bt cotton
soil
Bt insecticidal protein
persistence
