摘要
目的探讨汉族人群中外周血儿茶酚氧位甲基转移酶（COMT）基因表达与强迫症的关系。方法采用实时定量逆转录-聚合酶链反应技术检测35例强迫症首次发病患者（强迫症组）与31名健康对照（对照组）外周血COMT基因表达水平；使用美国精神障碍诊断与统计手册第4版轴Ⅱ诊断结构式临床访谈问卷（SCID-Ⅱ）评估强迫症与强迫性人格障碍的共病情况。结果强迫症组COMT基因表达水平明显低于对照组（t=2．56，P〈0．05），下调约32％；无强迫人格患者（15例）与对照组间COMT基因表达水平的差异有统计学意义（t=2．41，P〈0．05），而伴强迫人格患者（20例）与对照组的差异无统计学意义（t=1．50，P〉0．05）；伴强迫人格与无强迫人格的强迫症患者COMT基因表达水平分别下调14％和56％；COMT表达水平与症状严重度无显著相关（r=0．09，P〈0．05）。结论COMT基因表达下调可能与强迫症发病有关；伴或无强迫人格患者的遗传机制可能存在差异。
Objective To explore the association of peripheral catechol-O-methyl-transferase （COMT） gene expression and obsessive-compulsive disorder （OCD） in Han Chinese. Methods Peripheral blood samples were collected from individuals with OCD （ n = 35 ） and age- and sex-matched healthy subjects （n = 31 ）. Relative COMT gene expression levels were examined with real-time quantitative reverse transcription PCR in peripheral blood of all the subjects. Structured clinical interview for the 4th Edition of Diagnostic and Statistical Manual of Mental Disorder axis Ⅱ （ SCID- Ⅱ ） was used to assess the co-morbidity of OCD and obsessive-compulsive personality disorders （OCPD）. The Yale-Brown Obsessive-Compulsive Scale （Y-BOCS） was used to evaluate the severity of obsessive compulsive symptoms. Results The COMT gene expression levels were significantly decreased in the OCD group compared with healthy control group （t = 2. 56, P 〈 0. 05）. The OCD patients showed a 32% down-regulation of COMT gene expression. A significant lower COMT gene expression was observed in non-OCPD patients （ n = 15 ） in comparison with healthy controls （ t = 2. 41, P 〈 0. 05 ） , while there was no significant difference between OCPD patients （ n = 20） and healthy controls （ t = 1.50, P 〉 0. 05）. The rates of COMT gene expression down-regulation in OCD patients with OCPD and ones without OCPD were 14% and 56% respectively. There was no significant correlation between COMT expression level and the Y-BOCS score （ r = 0. 09, P = 0. 62）. Conclusions These results suggest that COMT gene expression down-regnlation is associated with OCD, and the OCD patients with and without OCPD may have different genetic background.
出处
《中华精神科杂志》
CAS
CSCD
北大核心
2009年第4期220-223,共4页
Chinese Journal of Psychiatry
基金
上海市卫生局青年科研基金资助项目（044Y26）
关键词
强迫症
强迫型人格障碍
儿茶酚氧位甲基转移酶
基因表达
Obsessive-compulsive disorder
Compulsive personality disorder
Catechol Omethyl transferase
Gene expression
