摘要
对不同取代度的纤维素月桂酸酯薄膜材料进行酶解处理,并运用高效液相色谱、凝胶渗透色谱(GPC)和扫描电镜(SEM)分别测定和观察了酶解过程产生的葡萄糖、降解前后的相对分子质量(Mr)及薄膜材料表观形貌。随着取代度(Ds)的降低,酶解液中的葡萄糖含量逐渐增加,葡萄糖得率也明显增加。GPC分析结果显示,样品经过酶解处理后其数均相对分子质量(Mn)减少,重均相对分子质量(Mw)增大,说明该类材料的酶降解主要是小分子质量成分的瓦解和流失。SEM图片中的薄膜表面均有斑点和凹洞出现。上述结果说明所得纤维素衍生物具有一定的酶可降解性。
A different assay was developed based on the utilization of ester enzyme and cellulolytic enzymes rather than dynamically mixed cultures found in the natural environment to degradate cellulose laurate films. The extent of biodegradation was followed by monitoring glucose production using HPLC. As the degree of substitution （ Ds ） decreased, the rate of biodegradation rates increased. After enzymatic degradation, the decrease of number-average molecular mass （Mn ） and the increase of weightaverage molecular mass （Mw ） denoted that the degradation occurred mainly from the collapse of low-molecular-mass constituents. Preferential attack of the microorganisms was observed on the face of the cellulose laurate film in SEM images. Collectively, this work indicated that the cellulose ester films with lower Ds showed definite biodegradability.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2009年第B10期101-104,共4页
Chemistry and Industry of Forest Products
基金
国家863计划资助(2007AA100704)
国家林业局948创新重点项目(2006-4-C03)
"十一五"国家科技支撑计划资助(2006BAD18B10)
关键词
取代度
纤维素酯
酶解
生物降解性
degree of substitution
cellulose ester
enzymatic degradation
biodegradation
