摘要
目的:探索细胞生长因子CTGF,PDGF和VEGF在臀肌挛缩带中的表达情况及其对臀肌挛缩症(GMC)的发病作用.方法:收集臀肌挛缩症患者挛缩带和病变旁肌肉的病理标本,运用逆转录-聚合酶链式反应(RT-PCR)、免疫组化和Western Blot技术检测细胞生长因子CTGF,PDGF和VEGF在病理组织中的表达情况.结果:细胞生长因子CTGF,PDGF和VEGF表达定位于成纤维细胞和血管内皮细胞的胞质中,并且呈强阳性表达,其在mRNA水平分别上调了3.5倍、5.8和2.7倍(P<0.05).蛋白水平的表达情况与mRNA一致,分别上调3.9倍、6.2倍和2.9倍(P<0.05).结论:细胞生长因子CTGF,PDGF和VEGF的表达上调可能是引起挛缩带产生的重要机制.
AIM：To explore expression and effect of CTGF,PDGF and VEGF on gluteal muscle contracture（GMC）.METHODS：Harvest contraction band and adjacent muscle from GMC patients and explore the expression of CTGF,PDGF and VEGF using immunohistochemistry,reverse transcription and polymerase chain reaction（RT-PCR）and Western Bolt analysis.RESULTS：CTGF,PDGF and VEGF was intensely expressed in fibroblast cells and vascular endothelial cells.The expression of those increased 3.5-fold,5.8-fold and 2.9-fold at mRNA level,respectively（P〈0.05）.Same changes were found in protein level,they increased 3.9-fold,6.2-fold and 2.9-fold,respectively（P〈0.05）.CONCLUSION：Increased expression of CTGF,PDGF and VEGF may initiate and propagate the fibrotic cascade in the gluteal muscles of GMC patients.
出处
《第四军医大学学报》
CAS
北大核心
2009年第19期1961-1963,共3页
Journal of the Fourth Military Medical University
