摘要
目的:研究中药蟾酥的脂溶性化学成分。方法:采用硅胶柱色谱、制备液相等技术分离纯化单体化合物,并根据理化性质及光谱数据鉴定结构。结果:分离得到8个甾体类化合物,并利用1H-NMR,13C-NMR以及文献比较的方法,分离鉴定了它们分别为:华蟾毒精(cinobufagin,1),脂蟾毒配基(resibufagenin,2),蟾毒灵(bufalin,3),蟾毒它灵(bufotalin,4),南美蟾毒精(marinobufagin,5),华蟾毒它灵(cinobufotalin,6),12β-羟基-蟾毒灵(12β-hydroxyl-bufalin,7),5,7β-二羟基-脂蟾毒配基(5,7β-dihydroxyl-resib-ufagenin,8)。结论:其中化合物7-8为属中首次发现。
Objective： To investigate the chemical constituents of traditional Chinese medicine Chansu. Methods： Their chemical constituents were isolated and purified by various chromatographic methods and preparative HPLC, and then their structures were identified by physicochemical properties and spectral analysis. Results： Eight known compounds were identified as cinobufagin（1）. resibufagenin（2）, bufalin（3）, bufotalin（4）, marinobufagin（5）, cinobufotalin（6）, 12β-hydroxyl-bufalin（7）,5, 7β-dihydroxyl-resibufagenin （8）. Conclusion： Among them, compounds 7- 8 were isolated from Chansu for the first time.
出处
《现代生物医学进展》
CAS
2009年第18期3519-3522,共4页
Progress in Modern Biomedicine
关键词
蟾酥
蟾蜍甾烯
结构鉴定
Veneum bufonis
Bufadienolides
Structural identification
