摘要
建立高效液相色谱法测定４种八角莲中鬼臼毒素含量的方法，考察不同溶剂及提取方法对含量测定结果的影响。方法Ｈｅｗｌｅｔ－Ｐａｃｋａｒｄ１１００高效液相色谱仪，ＷｈａｔｍａｎＰａｒｔｉＳｈｅｒｅＣ１８色谱柱，流动相：甲醇－０．１ｍｏｌ／Ｌ磷酸氢二钾（５０∶５０），检测波长：２９０ｎｍ。样品用８０％的甲醇水溶液超声波振荡提取结果标准曲线方程Ｙ＝０．２２５＋４３８．９５Ｘ，ｒ＝０．９９９７（ｎ＝３）。结论超声波法优于传统的回流提取法，本方法简便快速，准确度高。
PURPOSE To develop a method for determination of podophyllotoxin in Dysosma plants. Solvents and methods used to extract was tested. METHODS A high performance liquid chromatography method was used with Whatman PartiShere C 18 . The mobile phase was methanol 0.1mol/L K 2HPO 3(pH≈7.5)(50∶50). Flow rate was 1.0ml/min. Detection wavelength was at 290nm. The plants were extract by ultrasonic method with 80% methanol. RESULTS The linear range was from 2.6 to 13.0μg/ml. The standard curve equation was Y =0.225+438.95 X and the relevant coefficient was 0.9997( n =3). CONCLUSIONS This method was proved to be accuricy and simple.
出处
《上海医科大学学报》
CSCD
1998年第6期452-453,共2页
Journal of Fudan University(Medical Science)
关键词
鬼臼毒素
八角莲
高效液相色谱法
Podophyllotoxin
Dysosma versipellis (Hance) M. Cheng
D. veitchii (Hemsl. et Wils) Fu
D. guangxinesis Y. S. Wang
D. difformis (Hemsl. et Wils) Woods
high performance liquid chromatography
