摘要
探讨肝细胞癌(HCC)与 p53基因突变的关系。方法采用聚合酶链反应-限制性片段长度多态性分析技术,检测 HCC p53基因第7外显子点突变,研究来自黄曲霉素 B1(AFB1)污染严重的南宁地区的 HCC 标本55例。结果 34例 HCC p53基因249密码子有集中的点突变,突变频率为62%(34/55)。伴乙型肝炎表面抗原(HBsAg)阳性组 p53基因突变率62.5%,HBsAg 阴性组 p53基因突变率57%;伴 HCC 转移组 p53基因突变率89%,未转移组 p53基因突嗖率49%;高度分化、中度分化和低度分化的 HCC p53基因的突变率分别为17%、56%和82%。p53基因突变的 HCC 病人1年、3年和5年生存率为53%、29%和15%;p53基因不突变的 HCC 1年、3年和5年生存率分别为86%、62%和43%。结论 HCCp53基因249密码子出现突变热点可能是 AFB1和乙型肝炎病毒(HBV)感染协同作用的结果;p53基因突变与 HCC 的发生、发展及预后有关。
Objective To study the relationship between hepatocellular carcinoma (HCC) and p53 gene mutation. Methods Specimens of 55 HCC cases from Nanning prefecture of high aflatoxin B1 exposure were studied by polymerase chain reaction and restrictive fragment polymorphism analysis to examine the mutation the exon 7 of p53 gene.Results 61.8% of the cases were found to have mutation at codon 249 of p53 gene;62.5% for the HCCs with HBsAg positive,and 57.1% for those with HBsAg negative,88.9% for those with metastasis,and 48.7% for those without.The mutation rate of p53 gene was 16.7%,55.6% and 81.8% in the well-,mod- erately-and poorly-differentiated HCC respectively.For patients with the mutation of p53 gene,the survival rate of 1-year,3-year and 5-years was 52.9%,29.4% and 14.7% respectively,while for those with no mutation,the rate was 85.7%,61.9% and 42.9% respectively.Conclusion The results indicated that the hot-spot mutation at the codon 249 of p53 gene is a likely synergistic outcome of aflatoxin B1 and Hepatitis B virus (HBV) infection.And further study indicated that p53 gene mutation was correlated with the genesis and the development and the prognosis of HCC.
出处
《中华普通外科杂志》
CSCD
1998年第6期326-328,共3页
Chinese Journal of General Surgery
